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1.
Chinese Journal of School Health ; (12): 138-141, 2024.
Artigo em Chinês | WPRIM | ID: wpr-1011409

RESUMO

Objective@#To analyze the relationship between the risk of tuberculosis outbreaks in schools and the visit interval of index cases, so as to provide a scientific reference for predicting the risks of tuberculosis outbreak and making preventive measures.@*Methods@#A total of 630 index cases from school tuberculosis outbreaks were studied during January, 2015 to December, 2022. Data on demographics, consultation history, etiological diagnosis, and methods of detection were collected. Restricted Cubic Splines (RCS), unconditional Logistic regression, and the receiver operating characteristic curve (ROC curve) were used for analysis.@*Results@#The RCS fitted curve showed that the risk of a tuberculosis outbreak linearly increased when the consultation interval for etiologically negative patients exceeded 5.79 days, or for etiologically positive patients exceeded 8.37 days. After multi factor adjustment, for every additional day in the visit interval of the index case, the odds ratio ( OR ) value for a high risk outbreak was 1.10 (95% CI =1.07-1.13)( P <0.05). When analyzed by tertiles of visit intervals, compared to an interval of <14 days, the OR values (95% CI ) for high risk outbreaks in schools with intervals of 14-<28 days and ≥28 days were 10.32(3.04-35.10) and 82.58( 28.42 -239.95), respectively( P <0.01), indicating a trend of increasing outbreak risk with longer visit intervals. Based on the ROC curve analysis, the optimal threshold for predicting a high risk school tuberculosis outbreak was 23.5 days, with an area under the curve ( AUC ) of 0.93 (95% CI =0.89-0.98).@*Conclusion@#An extended visit interval of index cases is a good early warning indicator for high risk tuberculosis outbreaks in schools and could be considered a key factor in early intervention and risk control strategies.

2.
J. venom. anim. toxins incl. trop. dis ; 28: e20210116, 2022. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1375812

RESUMO

Background: Conopeptides from cone snail venom have aroused great interest related to the discovery of novel bioactive candidates, due to their excellent prospects for the treatment of various health problems such as pain, addiction, psychosis and epilepsy. In order to explore novel biopeptides, we investigated the structure and function of five novel conopeptides isolated from the venom of Conus marmoreus from South China Sea. Methods: C. marmoreus crude venom was prepared, fractionated and purified by HPLC system. The primary sequences of the five novel disulfide-poor conopeptides Mr-1 to Mr-5 were identified by comprehensive analysis of de novo MALDI-TOF tandem mass spectrometry and Edman degradation data. In order to investigate their function, these five conopeptides were synthesized by Fmoc-SPPS chemistry, and their biological effects at several heterologous rat nicotinic acetylcholine receptor (nAChR) subtypes (α1β1δε, α3β2, α3β4, α4β2) were determined by electrophysiological technique. Results: Five novel disulfide-poor conopeptides were identified and named as follows: Mr-1 (DWEYHAHPKPNSFWT), Mr-2 (YPTRAYPSNKFG), Mr-3 (NVIQAPAQSVAPP NTST), Mr-4 [KENVLNKLKSK(L/I)] and Mr-5 [NAVAAAN(L/I)PG(L/I)V]. None of them contains a disulfide bond. The sequences of conopeptides Mr-2 to Mr-5 do not belong to any category of the known disulfide-poor conopeptides. No significant activity against the above nAChR subtypes were observed for the five conopeptides at 100 µM. Conclusion: We purified and structurally characterized five novel disulfide-poor conopeptides from C. marmoreus crude venom and first investigated their nAChR inhibitory effects. This work expanded our knowledge on the structure and function of disulfide-poor conopeptides from this cone snail venom.(AU)


Assuntos
Animais , Conotoxinas/isolamento & purificação , Dissulfetos/efeitos adversos , Venenos de Moluscos , Espectrometria de Massas
3.
Chinese Journal of School Health ; (12): 1781-1784, 2021.
Artigo em Chinês | WPRIM | ID: wpr-906804

RESUMO

Objective@#To analyze delay in student pulmonary tuberculosis(PTB) case finding and associated factors in Suzhou, and to provide a reference for tuberculosis outbreak prevention and control in schools.@*Methods@#A total of 1 148 students with PTB who registered and were treated in Suzhou from 2011 to 2020 were included. Kruskal Wallis H test, 2 test and Cochran Armitage trend test were used to analyze the time trend of case finding delay. Logistic regression was used to analyze the correlation between admission characteristics and case finding delay.@*Results@#Among the students with PTB, a total of 569 cases were found to be delayed. The rate of delay was 49.6%, and the median delay time was 26(11-49) days. From 2011 to 2020, the difference in case finding interval of students with PTB was statistically significant( Hc=54.62, P <0.05), and the difference in case finding rate was also statistically significant( χ 2=53.69, P <0.05). The rate of delay fluctuated, with an overall upward trend over time( Z=-3.67, P < 0.05). Clinical consultation( OR=5.57, 95%CI =1.91-16.27), positive etiology ( OR=1.46, 95%CI =1.14-1.86) were positively correlated with case finding delay(all P <0.05).@*Conclusion@#There are significant delays in case finding among students with PTB in Suzhou. Clinical consultation and positive etiology are associated with case finding delay. In response to the growing problems in daily school tuberculosis prevention and control, multiple departments should cooperate to implement relevant measures and to reduce the occurrence of case finding delay.

4.
Chinese Acupuncture & Moxibustion ; (12): 734-738, 2019.
Artigo em Chinês | WPRIM | ID: wpr-776273

RESUMO

OBJECTIVE@#To evaluate the effectiveness in treatment of chronic neck pain with the direct moxibustion of small moxa cone and explore the dose-effect relationship in treatment of chronic neck pain with different small moxa cones.@*METHODS@#A total of 120 patients with chronic neck pain were randomized into a 3-moxa-cone group, a 5-moxa-cone group, a 7-moxa-cone group and a sham-moxibustion group, 30 cases in each one. Fengchi (GB 20), Tianzhu (BL 10), Jingbailao (EX-HN 15), Jianzhongshu (SI 15) and Jianjing (GB 21) were selected in each of the groups. The direct moxibustion with 3, 5 and 7 moxa cones as well as the sham-moxibustion therapy were provided successively in each of the above groups. In the sham-moxibustion group, the lower 1/3 section of moxa cone (about 1.5 mm in length) was soaked in oil before used in treatment. In each group, the treatment was given twice a week, for 10 treatments totally. Separately, before treatment, after treatment and in 1-month follow-up, the scores of the Northwick Park neck pain questionnaire (NPQ), the scores of McGill pain questionnaire (MPQ) and the local pressure pain threshold (PPT) were observed in each group. After treatment and in 1-month follow-up, the therapeutic effects were evaluated in each group.@*RESULTS@#After treatment and in 1-month follow-up, NPQ scores and MPQ scores in the 3-moxa-cone group, the 5-moxa-cone group and the 7-moxa-cone group were reduced as compared with those before treatment separately (all 0.05). After treatment and in 1-month follow-up, NPQ scores and MPQ scores in the 3-moxa-cone group, the 5-moxa-cone group and the 7-moxa-cone group were lower as compared with the sham-moxibustion group separately (all 0.05).@*CONCLUSION@#The direct moxibustion therapy with different small-moxa-cones effectively relieves chronic neck pain. There is a trend of improvement of the therapeutic effects with increase of the numbers of moxa cones.


Assuntos
Humanos , Pontos de Acupuntura , Moxibustão , Cervicalgia , Terapêutica , Medição da Dor , Inquéritos e Questionários
5.
Rev. bras. psiquiatr ; 40(3): 325-334, July-Sept. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-959245

RESUMO

Post-stroke depression (PSD) is a very common complication that leads to increased physical disability, poor functional outcome, and higher mortality. Therefore, early detection and treatment are very important. Since there are currently no specific guidelines for this disorder in China, the purpose of this study was to develop PSD guidelines and provide suggestions for clinicians and related workers.


Assuntos
Humanos , Acidente Vascular Cerebral/psicologia , Transtorno Depressivo/diagnóstico , Transtorno Depressivo/terapia , Psicoterapia , Fatores de Tempo , Índice de Gravidade de Doença , China/epidemiologia , Fatores de Risco , Guias de Prática Clínica como Assunto , Sobreviventes/psicologia , Depressão/etiologia , Transtorno Depressivo/tratamento farmacológico , Transtorno Depressivo/epidemiologia , Autorrelato , Reabilitação do Acidente Vascular Cerebral/normas , Antidepressivos/uso terapêutico
6.
Journal of Acupuncture and Tuina Science ; (6): 167-170, 2018.
Artigo em Chinês | WPRIM | ID: wpr-712668

RESUMO

Objective:To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque (CV 8) for preventing spleenstomach disharmony caused by venous analgesia pump.Methods:A total of 120 post-surgery patients using venous analgesia pump and also conforming to the inclusion criteria were randomized into 2 groups by the random number table.There were 58 patients in the control group who received conventional post-surgery nursing care,and there were 62 patients in the treatment group who received acupoint sticking at Shenque (CV 8) on the basis of conventional nursing care.After treatment,the incidences of postoperative nausea and vomiting,and abdominal bloating were measured.Results:The total incidence of nausea,vomiting and abdominal bloating in the treatment group was 11.3%,versus 39.7% in the control group,and the difference of the incidence between the two groups showed a statistical significance (P<0.05).In comparison of the severity of spleen-stomach dishormony which happened during 72 h after surgery,cases in the treatment group showed lower severity than those in the control group (P<0.05).Conclusion:Acupoint sticking at Shenque (CV 8) can effectively prevent spleen-stomach disharmony caused by venous analgesia pump,which will alleviate sufferings of the patients.

7.
Chinese Journal of Tissue Engineering Research ; (53): 2227-2232, 2018.
Artigo em Chinês | WPRIM | ID: wpr-698687

RESUMO

BACKGROUND:Various surface modification techniques have been utilized as an attempt to improve the osteointegration of Ti-based implants, which is a hotspot research concerning the artificial joint prosthesis. OBJECTIVE:To evaluate the effect of silicon doped zirconia film on the titanium surface on relevant factors of osteoblast-like MG63 cells. METHODS:Silicon doped zirconia and zirconia films were respectively prepared on the titanium surface by cathodic arc deposition. Osteoblast-like MG63 cells were cultured on silicon doped zirconia film, zirconia film and pure titanium, respectively. After 1, 4, 7, 10 days of incubation, samples were collected for assay. Osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL) at mRNA and protein levels were detected using real-time PCR and ELISA, respectively. RESULTS AND CONCLUSION:(1) At 1 day of inoculation, there was no significant difference in the OPG mRNA and protein expression among the three groups. At 4 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group (P<0.05), but there was no significant difference between the former two groups. At 7 and 10 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film growp was highest among the three groups (P<0.05). (2) At 1 day of inoculation, there was no significant difference in the RANKL mRNA and protein expression among the three groups. At 4 and 7 days of inoculation, the expression of RANKL mRNA and protein in the silicon doped zirconia film group was significantly lower than that in the other two groups (P<0.05). At 10 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group (P<0.05), but there was no significant difference between the former two groups. To conclude, silicon doped zirconia film fabricated by cathodic arc deposition can enhance the expression of OPG and reduce the expression of RANKL at the same time.

8.
Medical Journal of Chinese People's Liberation Army ; (12): 1072-1077, 2017.
Artigo em Chinês | WPRIM | ID: wpr-694060

RESUMO

Objective To explore the effects of gravitational traction and target regulation of caspase3 on the degenerative intervertebral disc cells in rabbits.Methods Rabbits nucleus pulposus cells transfected with caspase3 siRNA or negative control siRNA were incubated in serum-starved medium for 6 hours and 48 hours.The expression of caspase3 siRNA and cell apoptosis were analyzed by RT-qPCR and Annexin V-fluorescein staining.In order to create intervertebral disc degeneration model,the right anterior side of the annulus fibrosus of lumbar vertebrae of 35 rabbits were damaged by 16-gauge needle.After confirming the success of modeling,35 animal models were randomized into 3 groups:model group (n=10),negative siRNA group (n=10) and caspase3 siRNA group (n=5).Either negative control siRNA or caspase3 siRNA was injected into the center of nucleus pulposus using a 26-gauge needle from the left anterior side,while the model group received no injection.Nucleus pulposus tissue of 5 rabbits selected randomly from every group after 48 hours were analyzed by PCR and Western blotting for caspase3 mRNA and protein expressions.Half of the casepase3 siRNA group were selected randomly and received a routine gravitational traction using a model of our own design,30min per day for 2 weeks,while other groups received no treatment.TNF-o and IL-1β expression levels and histopathological observations were performed after intervention.Results Expression of caspase3 mRNA in rabbit nucleus pulposus cells transfected with caspase3 siRNA decreased obviously in serum-starved medium,and the apoptosis rate of cells cultured in serum-starved medium decreased significantly (P<0.05).Caspase3 mRNA and caspase3 protein expression of nucleus pulposus injected by caspase3 siRNA down-regulated in the caspase3 siRNA group.Compared with model group and negative control siRNA group,TNF-α and IL-1β expression levels of caspase3 siRNA traction group decreased significantly (P<0.05),but there was no statistical significance compared with caspase3 siRNA group (P>0.05).Pathological observation revealed that viable cell number and extracellular matrix contents increased and collagenous fibers arranged regularly in caspase3 siRNA traction group.Conclusion Gravitational traction and target regulation of caspase3 can prevent apoptotic cell death and delay early intervertebral disc degeneration.

9.
China Journal of Chinese Materia Medica ; (24): 3991-4000, 2014.
Artigo em Chinês | WPRIM | ID: wpr-310952

RESUMO

<p><b>OBJECTIVE</b>To establish an HPLC fingerprint to evaluate the quality of Polygalae Radix, root xylem, and those collected in different growth ages or harvest time.</p><p><b>METHOD</b>Separation was performed at 30 °C on a Kromasil C18 column (4.6 mm x 250 mm, 5 μm); the mobile phases was acetonitrile and 0.05% H3PO4 water in the gradient elution; the flow rate was set at 1.0 mL · min(-1) and the detection wavelength at 314 nm; the quality discriminant analyses were accomplished by means of similarity analysis, cluster analysis, principal component analysis and neural network model.</p><p><b>RESULT</b>In 26 batches of Polygalae Radix, 24 batches fingerprint similarities were above 0.8. In 5 different growth or harvest time batches, 4 batches were above 0.8; in 8 batches root xylem samples, the similarities were all above 0.875. The similarity analysis was in accord with the quality discriminant analysis of cluster analysis, principal component analysis and neural network model.</p><p><b>CONCLUSION</b>Fingerprint combined with chemical pattern recognition technique can effectively evaluate the quality of Polygalae Radix. The active substance species are all similar in cultivated, wild, different growth or harvest time Polygalae Radix and polygala root xylem, but the chromatography peak areas are different. The effective material contents are similar between wild and cultivated Polygalae Radix, but each chromatographic peak area of the root xylem is much smaller than that of Polygalae Radix. The chemical substance accumulation mainly depends on harvest month, but little growth time in Polygalae Radix.</p>


Assuntos
Cromatografia Líquida de Alta Pressão , Métodos , Medicamentos de Ervas Chinesas , Química , Raízes de Plantas , Química , Classificação , Polygala , Química , Classificação , Controle de Qualidade
10.
Acta Pharmaceutica Sinica ; (12): 1029-1033, 2014.
Artigo em Chinês | WPRIM | ID: wpr-299172

RESUMO

The paper reported an investigation of the pharmacokinetics of SN-38 (7-ethyl-10-hydroxy-camptothecin) in rats and the tissue distribution in mice after injection of irinotecan hydrochloride nanoparticles (CPT-11) via tail veins. An LC-MS/MS method was established to determine the concentrations of SN-38 in whole blood of rats and in different tissues of mice. The pharmacokinetics and tissue distribution of SN-38 were compared after the intravenous injection of CPT-11 NPs and CPT-11 solution. Compared with irinotecan solution, the elimination half-life of SN-38 was prolonged from 2.17 h to 2.67 h after the intravenous injection of CPT-11 NPs, but its AUC had little change. After the injection of CPT-11 NPs in mice, over time, the concentrations of CPT-11-metabolized SN-38 in CPT-11 NPs were significantly higher in the whole blood, colon and lungs than those in CPT-11 solution, followed by in the spleen and liver, but those in the heart and brain had no change. However, the amount of SN-38 in the kidneys was reduced with time. CPT-11 NPs could prolong SN-38's (one of its metabolites) blood circulation time in rats and significantly increased the concentration of CPT-11-metabolized SN-38 in the whole blood, colon and lungs of mice. CPT-11 NPs made SN-38 efficiently target-bind to the colon and lungs of mice.


Assuntos
Animais , Camundongos , Ratos , Antineoplásicos Fitogênicos , Farmacocinética , Camptotecina , Farmacocinética , Cromatografia Líquida , Colo , Metabolismo , Meia-Vida , Injeções Intravenosas , Pulmão , Metabolismo , Nanopartículas , Espectrometria de Massas em Tandem , Distribuição Tecidual
11.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 356-360, 2013.
Artigo em Chinês | WPRIM | ID: wpr-343666

RESUMO

<p><b>OBJECTIVE</b>To analyze the prevalence and risk factors of multiple musculoskeletal disorders (MSDs) in auto workers and the associations between MSDs at different sites.</p><p><b>METHODS</b>A cross-sectional survey was conducted in 3998 workers, who were selected from a Chinese auto corporation by cluster random sampling, using the revised Nordic MSDs standard questionnaire; 3800 completed questionnaires were returned. Multinomial logistic regression analysis was performed to assess the risk factors for multiple MSDs. The logbinomial model was used to calculate the prevalence ratios (PRs) of MSDs at different sites and evaluate the associations between MSDs at different sites.</p><p><b>RESULTS</b>Of the 3800 subjects, 2452 (64.5%) had MSDs at two or more sites, and 469 (12.3%) had MSDs at one site. The PRs varied from 1.5 to 6.7, with significant differences among different sites (P < 0.01). Relatively close associations were found between the MSDs at neck and shoulders, back and shoulders/waist, elbows and wrists/hands, waist and neck, wrists/hands and waist, hip and waist, knees and waist, and ankles/feet and elbows. The multinomial logistic regression analysis indicated that the highest risk factor for MSDs was poor posture, including often working in an uncomfortable posture, neck bending forward, and neck twisting (ORs = 3.39, 1.93, and 1.38), followed by labor organization, in which break between tasks could decrease the risk of MSDs at three or more sites to 31%, staff shortage, which could increase the risk of MSDs by 75%, and pushing and pulling heavy objects (> 20 kg) (OR = 1.76).</p><p><b>CONCLUSION</b>Most auto workers with MSDs have multiple sites affected, and there are high associations between the MSDs at different sites. The major risk factors for multiple MSDs in auto workers include poor posture, labor organization, and heavy physical labor.</p>


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Automóveis , Estudos Transversais , Modelos Logísticos , Doenças Musculoesqueléticas , Epidemiologia , Doenças Profissionais , Epidemiologia , Fatores de Risco , Inquéritos e Questionários
12.
Chinese Journal of Pediatrics ; (12): 443-447, 2013.
Artigo em Chinês | WPRIM | ID: wpr-275691

RESUMO

<p><b>OBJECTIVE</b>To analyze the clinical and SLC2A1 gene mutation characteristics of glucose transporter type 1 deficiency syndrome.</p><p><b>METHOD</b>The detailed clinical manifestations of six cases were recorded. The laboratory tests including EEG, MRI, blood chemistry, and lumbar puncture were performed. SLC2A1 gene mutations were analyzed by PCR, DNA sequencing and multiplex ligation-dependent probe amplification (MLPA).</p><p><b>RESULT</b>Patient 1, 2 and 3 had classical clinical symptoms including infantile onset seizures, development delay. Patient 4, 5 and 6 had non-classical clinical symptoms including paroxysmal behavior disturbance, weakness, ataxia, lethargy, especially after fasting or exercise, without severe seizures. The plasma glucose levels were normal. The CSF glucose levels decreased in all the six cases, ranged from 1.10 mmol/L to 2.45 mmol/L, the mean level was 1.68 mmol/L. The CSF glucose/plasma glucose ratios decreased, ranged from 0.16 to 0.51, the mean ratio was 0.34. Four patients had normal EEG. Two patients had focal and diffuse epileptiform discharge, and one of them also had paroxysmal occipital or generalized high-amplitude slow waves during awake and sleep time. MRI abnormalities were found in three patients, patient 1 with mild brain atrophy, patient 3 with bilateral ventricle plump, and patient 4 with high signals in T2 in the frontal and occipital white matter, interpreted as hypomyelination. SLC2A1 gene mutations were found in six cases. Patient 1 has large scale deletion in exon 2. In patient 2 to 6, the mutations were c.741 G>A (E247K), 599delA, 761delA, c.1148 C>A (P383H), c.1198 C>T (R400C) respectively. Two patients were treated with ketogenic diet. The seizures disappeared and development became normal. Three patients responded to frequent meals with snacks. One patient refused any treatments, the symptoms continued to exist.</p><p><b>CONCLUSION</b>The clinical manifestations of glucose transporter type 1 deficiency syndrome are varied. The common symptoms included infantile onset seizures and various paroxysmal events. These neurologic symptoms generally fluctuated and were influenced by factors such as fasting or fatigue. This feature could be a very important clue for the diagnosis of GLUT1-DS. Lumbar puncture is recommended in patients with episodic CNS symptoms especially after fasting. GLUT1-DS is a treatable neurometabolic disorder, early diagnosis and treatment may improve the prognosis of the patients.</p>


Assuntos
Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Biomarcadores , Encéfalo , Diagnóstico por Imagem , Patologia , Erros Inatos do Metabolismo dos Carboidratos , Diagnóstico , Genética , Terapêutica , Análise Mutacional de DNA , Dieta Cetogênica , Eletroencefalografia , Epilepsia , Diagnóstico , Genética , Terapêutica , Seguimentos , Transportador de Glucose Tipo 1 , Genética , Imageamento por Ressonância Magnética , Proteínas de Transporte de Monossacarídeos , Genética , Transtornos dos Movimentos , Diagnóstico , Genética , Terapêutica , Mutação , Genética , Radiografia
13.
Acta Pharmaceutica Sinica ; (12): 741-745, 2013.
Artigo em Chinês | WPRIM | ID: wpr-259556

RESUMO

The object of this study is to investigate the pharmacokinetic interaction of pioglitazone hydrochloride and atorvastatin calcium in healthy adult Beagle dogs following single and multiple oral dose administration. A randomized, cross-over study was conducted with nine healthy adult Beagle dogs assigned to three groups. Each group was arranged to take atorvastatin calcium (A), pioglitazone hydrochloride (B), atorvastatin calcium and pioglitazone hydrochloride (C) orally in the first period, to take B, C, A in the second period, and to take C, A, B in the third period for 6 days respectively. The blood samples were collected at the first and the sixth day after the administration, plasma drug concentrations were determined by LC-MS/MS, a one-week wash-out period was needed between each period. The pharmacokinetic parameters of drug combination group and the drug alone group were calculated by statistical moment method, calculation of C(max) and AUC(0-t) was done by using 90% confidence interval method of the bioequivalence and bioavailability degree module DAS 3.2.1 software statistics. Compared with the separate administration, the main pharmacokinetic parameters (C(max) and AUC(0-t)) of joint use of pioglitazone hydrochloride and atorvastatin calcium within 90% confidence intervals for bioequivalence statistics were unqualified, the mean t(max) with standard deviation used paired Wilcoxon test resulted P > 0.05. There was no significant difference within t1/2, CL(int), MRT, V/F. Pioglitazone hydrochloride and atorvastatin calcium had pharmacokinetic interaction in healthy adult Beagle dogs.


Assuntos
Animais , Cães , Feminino , Masculino , Administração Oral , Anticolesterolemiantes , Sangue , Farmacocinética , Área Sob a Curva , Atorvastatina , Sangue , Farmacocinética , Disponibilidade Biológica , Estudos Cross-Over , Interações Medicamentosas , Inibidores de Hidroximetilglutaril-CoA Redutases , Sangue , Farmacocinética , Hipoglicemiantes , Sangue , Farmacocinética , Distribuição Aleatória , Tiazolidinedionas , Sangue , Farmacocinética
14.
Acta Pharmaceutica Sinica ; (12): 940-945, 2013.
Artigo em Chinês | WPRIM | ID: wpr-259526

RESUMO

To investigate the pharmacokinetics of irinotecan hydrochloride (CPT-11) in rats and the tissue distribution of CPT-11 in mice after injection of irinotecan hydrochloride nanoparticles (CPT-11 NPs) via tail veins, separately, a LC-MS/MS method was established to determine the concentration of CPT-11 in whole blood of rats and in different tissues of mice. The pharmacokinetics and tissue distribution of CPT-11 were compared after the intravenous injection of CPT-11 NPs and CPT-11 solution. Compared with CPT-11 solution, the elimination half-life of CPT-11 was prolonged from 2.28 h to 3.95 h after the intravenous injection of CPT-11 NPs, and its AUC was 1.47 times than that of CPT-11 solution. After the injection of CPT-11 NPs in mice, the concentrations of CPT-11 loaded in CPT-11 NPs were significantly higher in the whole blood, colon and lungs than those in CPT-11 solution, but lower in the spleen, liver, kidney and heart, but the least in brain. CPT-11 NPs could improve CPT-11 's AUC, and help CPT-11 to reach long circulation activity.


Assuntos
Animais , Feminino , Masculino , Camundongos , Ratos , Antineoplásicos Fitogênicos , Sangue , Farmacocinética , Área Sob a Curva , Camptotecina , Sangue , Farmacocinética , Cromatografia Líquida de Alta Pressão , Injeções Intravenosas , Nanopartículas , Distribuição Aleatória , Ratos Sprague-Dawley , Espectrometria de Massas por Ionização por Electrospray , Distribuição Tecidual
15.
Chinese Journal of Stomatology ; (12): 562-566, 2012.
Artigo em Chinês | WPRIM | ID: wpr-260235

RESUMO

<p><b>OBJECTIVE</b>To explore the effect of DNAX-associated protein 12 (DAP12) pathway on the transformation from mouse monocytes RAW264.7 to osteoclasts induced by tensile strain.</p><p><b>METHODS</b>DAP12shRNA plasmid was constructed and introduced to RAW264.7 cells. Then we supplied tensile strain to RAW264.7 cells by four-point bending system. The mRNA or protein expression of DAP12, tartrate-resistant acid phosphatase (TRAP), tyrosine kinases Btk and Tec and nuclear facior of activated T cells 1 (NFATc1) was measured by reverse transcription PCR (RT-PCR) and Western blotting respectively.</p><p><b>RESULTS</b>The expression of DAP12 mRNA (0.112 ± 0.025) and protein (0.193 ± 0.015) both declined sharply after plasmid being introduced into monocytes RAW264.7 (P < 0.05). After silencing DAP12 expression in RAW264.7 cells by RNA interference, tensile strain-induced TRAP mRNA expression of RAW264.7 cells increased at 6 h (0.671 ± 0.031) and 12 h (0.800 ± 0.043) (P < 0.05), but it was weaker than non-RNA-interference-groups at each time point (P < 0.05). After silencing DAP12 expression in RAW264.7 cells by RNA interference, the expressions of Btk, Tec, NFATc1 increased as time passed (6, 12 h) (P < 0.05), but the expressions on corresponding time decreased sharply compared with those in control groups (P < 0.05).</p><p><b>CONCLUSIONS</b>DAP12 pathway play an important role in regulating osteoclast differentiation induced by tensile strain.</p>


Assuntos
Animais , Camundongos , Fosfatase Ácida , Genética , Metabolismo , Proteínas Adaptadoras de Transdução de Sinal , Genética , Metabolismo , Diferenciação Celular , Linhagem Celular , Regulação da Expressão Gênica , Inativação Gênica , Isoenzimas , Genética , Metabolismo , Monócitos , Biologia Celular , Metabolismo , Fatores de Transcrição NFATC , Metabolismo , Osteoclastos , Biologia Celular , Plasmídeos , Proteínas Tirosina Quinases , Metabolismo , RNA Mensageiro , Metabolismo , RNA Interferente Pequeno , Transdução de Sinais , Fosfatase Ácida Resistente a Tartarato , Resistência à Tração
16.
Biomedical and Environmental Sciences ; (12): 122-129, 2009.
Artigo em Inglês | WPRIM | ID: wpr-360688

RESUMO

<p><b>OBJECTIVE</b>To examine the effects of chlorogenic acid (CGA) on lipid and glucose metabolism under a high dietary fat burden and to explore the possible role of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) in these effects.</p><p><b>METHODS</b>Twenty male golden hamsters were randomly divided into CGA treatment group (n=10, given peritoneal injection of CGA solution prepared with PBS, 80 mg CGA/kg body weight daily), and control group (n=10, given PBS i.p. at the average volume of the treatment group). Animals in both groups were given 15% high fat diet. Eight weeks after treatment with CGA, the level of biochemical parameters in fasting serum and tissues and the expression of hepatic mRNA and protein PPAR-alpha were determined.</p><p><b>RESULTS</b>Eight weeks after treatment with CGA, the levels of fasting serum triglyceride (TG), free fatty acid (FFA), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), glucose (FSG), and insulin (FSI) were significantly lower in the GGA treatment group than in the control group. CGA also led to higher activity of hepatic lipase (HL), lower contents of TG and FFA in liver, and lower activity of lipoprotein lipase (LPL) in skeletal muscle. Furthermore, CGA significantly elevated significantly elevated the expression level of mRNA and protein expression in hepatic PPAR-alpha.</p><p><b>CONCLUSION</b>CGA can modify lipids and glucose metabolism, which may be attributed to PPAR-alpha facilitated lipid clearance in liver and improved insulin sensitivity.</p>


Assuntos
Animais , Cricetinae , Masculino , Glicemia , Ácido Clorogênico , Farmacologia , Gorduras na Dieta , Farmacologia , Regulação da Expressão Gênica , Glucose , Metabolismo , Hipolipemiantes , Farmacologia , Lipase , Metabolismo , Metabolismo dos Lipídeos , Mesocricetus , PPAR alfa , Genética , Metabolismo , RNA Mensageiro , Genética , Metabolismo , Aumento de Peso
17.
Chinese Journal of Medical Instrumentation ; (6): 79-82, 2009.
Artigo em Chinês | WPRIM | ID: wpr-329371

RESUMO

The basic theory of Higher Order Spectral Analysis and the most generally used Bispectrum are introduced in the paper. By certain experiments of EEG signal acquisition and bispectrum analysis, it is showed that the Higher Order Spectrum has an advantage over power spectrum, which is based on Second Order Statistics, in processing non-linear signal and restraining Gauss noise signal.


Assuntos
Eletroencefalografia , Métodos , Processamento de Sinais Assistido por Computador
18.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 596-600, 2008.
Artigo em Chinês | WPRIM | ID: wpr-315735

RESUMO

<p><b>OBJECTIVE</b>To understand the major causes of death in automobile foundry workers and investigate casting manipulations hazards to health.</p><p><b>METHODS</b>A cohort study of 3529 foundry workers registered in one big automobile factory in Shiyan city of China was performed. Standardized mortality ratios (SMRs) were calculated for the main causes of death by using Chinese national mortality rates as reference.</p><p><b>RESULTS</b>The cohort mortality was traced from 1980 to the end of 2005 with an accumulation of 84 999 person-years, revealed 265 deaths. The results of this study showed that the standardized mortality ratio for all subjects was 0.96 (95% CI: 0.85 approximately 1.08), which was very close to that expected on the basis of the China national mortality rates. The SMR increased with age, the SMR became greater than 1 in all groups of age 50 and higher. The results showed that malignant neoplasm (3.43%), accidents (1.16%), cerebro-vascular diseases (1.08%), cardio-vascular diseases (0.79%) were the first four illnesses that threatened workers' life span. Statistically significant mortality of malignant neoplasm (SMR = 7.87), accidents (SMR = 2.70), cardio-vascular diseases (SMR = 2.68) and digestive diseases (SMR = 2.79) were found in the foundry workers. The relative risk of malignant neoplasm for first line workers to assistant workers was 1.99 (P < 0.05).</p><p><b>CONCLUSION</b>The occupational hazards in foundry factory have harmful impact on the workers' health and life span.</p>


Assuntos
Feminino , Humanos , Masculino , Automóveis , Causas de Morte , China , Epidemiologia , Seguimentos , Metalurgia , Mortalidade , Doenças Profissionais , Mortalidade , Exposição Ocupacional , Estudos Retrospectivos
19.
Chinese Journal of Medical Instrumentation ; (6): 330-332, 2007.
Artigo em Chinês | WPRIM | ID: wpr-323260

RESUMO

Many researches have proved that auditory evoked potentials (AEP) can indicate the depth of anesthesia in the clinical practice. This paper introduces two methods of extracting AEP: the moving time average (MTA) model and the autoregressive model with exogenous input (ARX). In our study we used both the MTA model and the ARX model to extract AEP and the results show that using the ARX model can get the results significantly faster than using the MTA model, and the ARX model is more suitable for the real-time monitoring of the anesthetic depth.


Assuntos
Humanos , Anestesia , Métodos , Eletroencefalografia , Métodos , Potenciais Evocados Auditivos , Fisiologia , Monitorização Intraoperatória , Métodos , Processamento de Sinais Assistido por Computador
20.
Chinese Journal of Biotechnology ; (12): 514-519, 2007.
Artigo em Chinês | WPRIM | ID: wpr-327994

RESUMO

The principal component analysis (PCA) was applied to the data processing in training sets, the new principal components were then used as input data for support vector machine model. A prediction model for optimum pH of chitinase was established based on uniform design. When The regularized constant C, epsilon and Gamma were 10, 0.7 and 0.5 respectively, the calculated pHs fitted the reported optimum pHs of chitinase very well and the MAPEs (Mean Absolute Percent Error) was 3.76%. At the same time, the predicted pHs fitted the reported optimum pHs well and the MAE (Mean Absolute Error) was 0.42 pH unit. It was superior in fittings and predictions compared to the model based on back propagation (BP) neural network.


Assuntos
Animais , Humanos , Algoritmos , Quitinases , Química , Metabolismo , Concentração de Íons de Hidrogênio , Modelos Biológicos , Modelos Estatísticos , Redes Neurais de Computação , Análise de Componente Principal
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