RESUMO
Objective To explore effects of different doses of 60Co γ-ray irradiation on apoptosis of rat submandibular gland dells.Methods Wistar rats were randomly divided into four different radiotherapy dosage groups (0 G y,7.5 G y,15 Gy,and 22.5 Gy).Rats in each radiotherapy groups were irradiated by the required dose of γ-ray irradiation (0 Gy,7.5 Gy,15 Gy,22.5 Gy) at one time.Immunohistochemistry method and TdT-mediated dUTP nick end labeling (TUNEL) methods were used to detect expression of P53,caspase-3,and apoptosis of submandibular gland dells in rats.Results The expression levels of P53 and caspase-3 of rat submandibular cells were significantly increased in different radiotherapy dosage groups (7.5 G y,15 Gy,and 22.5 Gy) relative the 0 Gy dosage group.The apoptotic cells were more commonly seen in duct cells in different radiotherapy dosage groups,and the apoptotic cells were gradually increased with the increased radiotherapy dosages.Conclusions 60 Co γ-ray irradiation could lead to apoptosis of rat submandibular gland in the early stage.There is a dose-effect relationship between 60Co γ-ray irradiation and induced apoptosis of rat submandibular gland cells.
RESUMO
Objective To investigate the clinical efficacy of Budesonide spray atomization inhalation on ear-nose-pharyngeal-throat acute inflammation.Methods 120 patients with ear-nose-pharyngeal-throat acute inflammation were slected from April 2010 to April 2013 in Liuzhou People’s Hospital.They were randomly divided into control group(n=60 )and observation group (n=60 ).The patients in observation group were treated with budesonide spray atomization inhalation,while the patients in control group were treated with conventional ultrasonic aerosol inhalation,continous use for 5 days.Compare the improvement of throat pain,pharyngeal foreign body sensation,dyspnea,hoarseness and the average improving time after treatment in two groups. Results The total effective rate of throat pain,pharyngeal foreign body sensation,dyspnea,hoarseness and the average improving time in observation group was significantly better than that in control group,the difference was statistically significant(P<0.05 ).Conclusion Compared with conventional ultrasonic atomization inhalation,the efficacy of budesonide spray atomization inhalation on ear-nose-pharyngeal-throat acute inflammation is better.