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Objective:To investigate the effects of sodium aescinate on endothelin ( ET ) and calcitonin gene -related peptide ( CGRP) contents in the gerbils with cerebral ischemia/reperfusion injury ( CI/R) . Methods:The gerbil model of CI/R was prepared by bilateral common carotid arteries ligation for 10 min followed by 2-hour reperfusion. Sodium aescinate (i. p. , 10, 20 and 40 mg· kg-1 ) was administered once a day for 3 days before the operation and once every 1 h after the operation, respectively. The levels of ET and CGRP in brain tissue homogenate were determined by a radioimmunoassay method. Results:Sodium aescinate significantly in-hibited the level of ET (28. 69-37. 03 ng·L-1) in the brain tissue of gerbils with CI/R compared with the model group (P0. 05). Conclusion:Sodium aescinate has obvious protective effects a-gainst CI/R in gerbils, which may be due to its inhibitory action on ET levels in brain tissue.
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Objective To investigate the acute toxicity and anti-stress effects of Naoxinshu capsule (NC) in mice.Methods In acute toxicity experiment,mice were oral administered to determine the maximum tolerance dose of NC.In anti-stress experiments,mice were divided into 5 groups at random,namely NC high,medium and low dose groups (2.4,1.2 and 0.6 g/kg,respectively),Gypenosides group(0.75 g/kg) and control group (n=10).After seven days of oral administration,rope climbing time,loaded swimming time,anti-hypoxia time,and survival rate under high or low temperature tests were carried out,respectively.Results The maximum tolerance dose of NC was 72 g/kg.NC high,medium and low dose groups significantly prolonged the rope climbing time [is (9.5 ± 3.1) min,(6.0± 1.6) min,(4.1 ± 1.2) min],loaded swimming time [is (28.2± 6.8) min,(25.7± 10.3)min,(22.9± 8.0)min] and anti-hypoxia time (around 17 min),compared to the control group (P＜0.05 or P＜0.01).NC high dose group significantly improved the survival rate of mice (i.e.30％) under high or low temperature (P＜0.05).Conclusion NC shows good anti-stress effects,and no detectable acute toxicity in mice.
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OBJECTIVE To investigate the distribution of pathogenic bacteria in blood culture and their drug-sensitivity in our hospital in the past year.METHODS The blood samples were cultivated in the BacT/Alert 3D system and the isolated colonies were identified by the VITEK32 system or traditional routine procedures.The K-B method was carried out for the drug-sensitivity festing.RESULTS From the 716 blood samples received,we identified 109 strains of pathogens in which Gram-positive cocci accounted for 50.46%,Gram-negative bacilli for 44.04% and fungi for 5.5%.CONCLUSIONS There are many kinds of pathogens identified from blood culture and they have high resistance to many antibiotices.It is very important for doctors to get the results of blood culture in time in order to give the patients correct antibiotic therapy and to improve the cure rate and to control cross-infection in hospials.
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OBJECTIVE To understand the mechanisms,adverse effect and notices in use of quinalones.METHODS The adverse effect of quinalones used sinylely or in combination was collected and analyzed.RESULTS Serious central nerve system side-effects,phototoxicity,hepatotoxicity hemolytic anemia,uremia and so on could be found in few cases.Some quinolones could result in Q-T interval elongation inducing the severe ventricular dysrythmia.The serum glucose also could be affected when quinolones were used together with the medications for diabetes.CONCLUSIONS The key points for quinolones usage are rational use and paying attention to their safety.