RESUMO
Objective To establish the method of content determination of chlorogenic acid in Shuanghua eardrop. Methods High performance liquid chromatography (HPLC) was adopted with Kromasil C18 column (5 ?m,4.6 mm?250 mm) and acetonitrile-0.4% phosph acid solution (10∶85) as the mobile phase. The detective wavelength was 328 nm,the flow rate was 1.0 mL/min,column temperature was room temperature. Results Average recovery was 99.8%,relative standard error was 1.51% (n =5). Conclusion The linearity relationship of chlorogenic acid was good at the range of 0.102 4～0.061 44 ?g.
RESUMO
Objectve To study the effect of anti-swelling and alleviating pain of Huzhangtongluo liniment. Method Mice with xylol-induced auricular swelling : the mice of each group were administered three times per day for 3 days. The inflammed mice were killed and punched a patch on left and right ear in 8 mm diameter, the weight margin of two patch was the rate of swelling. Mice with formalin-induced podalic swelling:the inflammed mice of each group were administered after 4 hours, by dislocating their cervical vetebra. The mice posterior feet were cut at the same place and weighed, the weight margin of left and right foot was the rate of swelling. Rat with granulation induced by cotton embedded under dermis:the rat of each group were administered and then killed after 6 hours, the granulation tissue of rat was separated, dried in oven at temperature of 70 ℃ and weighed, the data of dry weight was calculated and processed by statistics. The pain threshold of mice stimulated by heat:the pain threshold of administered mice was determined at 30, 60, 90 min. The threshold value was calculated as 60 s if the mice had no action such as licking foot, jumping or lifting foot after 60 s. Result Huzhangtongluo liniment can obviously inhibit auricular swelling induced by xylol and podalic swelling induced by formalin as well as granulation induced by cotton embeded under dermis. The preparations can clearly enhance pain-threshold value of mice in hot-plate method. Conclusion Huzhangtongluo liniment has the action of anti-swelling and alleviating pain.