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1.
Biomedica. 2011; 27 (Jan.-Jun.): 57-61
em Inglês | IMEMR | ID: emr-110358

RESUMO

Frequency of Hepatitis B Surface Antigen [HBsAg] and Hepatitis C Virus Antibodies [Anti-HCV] among blood donors of Lahore and their association with blood group types. To study the frequency of Hepatitis B Surface Antigen [HBsAg] and Hepatitis C Virus Antibodies [Anti-HCV] in blood donors of Lahore and to assess the association with blood group types. The design of study will be cross sectional descriptive study. It was held in the Pathology Department, Nawaz Sharif Social Security Hospital, Lahore, during the period January, 2006 to December, 2008. A total of 16695 blood donors were screened for HBsAg and Anti-HCV by rapid test devices based on immuno-chromatographic technique following the instruction given by the manufacturer. In the present study, devices manufactured by Acon, USA were used. The specimens reactive on screening by devices were confirmed on ELISA. The results were subjected to chi-square analysis for determination of statistical difference between the values among different categories. Among 16695 blood donors, 467 [2.79%] were positive for HBsAg and 1326 [7.94%] were positive for Anti-HCV. The frequency of HBsAg was seen to decrease significantly [p < 0.01] from 2006 to 2008 [4.23% to 2.31%]. However, frequency of anti-HCV was seen to rise significantly [< 0.01] from 2006 [6.69%] to 2008 [7.82%]. Comparison of HBsAg and anti-HCV positivity among RhD positive and RhD negative donors showed that there was no significant difference for HBsAg positivity [2.79% vs 2.85%]. However, significantly higher number of RhD positive donors had HCV infection as compared to RhD negative donors [8.25% vs 3.66%]. High frequency of HCV infection in blood donors need implementation of strict screening policy for donors and public awareness campaigns about preventive measure to reduce the spread of this infection as well as other transfusion transmissible infections. Association of HCV infection with blood group types needs more studies to get more knowledge about this aspect


Assuntos
Humanos , Masculino , Feminino , Sistema ABO de Grupos Sanguíneos , Hepatite B , Hepatite C , Antígenos de Superfície da Hepatite B , Anticorpos Anti-Hepatite C , Doadores de Sangue , Estudos Transversais , Ensaio de Imunoadsorção Enzimática
2.
Biomedica. 2009; 25 (Jan.-Jun.): 56-58
em Inglês | IMEMR | ID: emr-100167

RESUMO

This study was carried out to find the frequency of Methicillin Resistant Staphylococcus aureus [MRSA] in a tertiary care hospital. This is a cross sectional descriptive study performed in Pathology Department, Nawaz Sharif Social Security Hospital, Multan Road, Lahore, during the period January, 2008 to December, 2008. Two hundred and thirty three consecutive, non-duplicate strains of Staphylococcus aureus were isolated from a variety of clinical specimens of pus/ pus swab, throat swab, sputum, urine, semen, ear swab, vaginal swab, blood, pleura/fluid and cerebrospinal fluid were studied for Methicillin resistance. Standard methodology using modfied Kirby- Baur disk diffusion method was adopted. Oxacillin [1 micro g disk] was used to detect Methicillin resistance. An inhibition zone of less than 10 mm was taken as indicative of MRSA. Out of 233 Staphylococcus aureus isolates, 81 [34.76%] were found to be Methicillin resistant. The yield of MRSA was highest from pus/ pus swab and sputum samples [40.0%], followed by throat swab [35.71%], urine, blood and pleural fluid [33.33%], semen [30.43%], vaginal swab [27.27%], ear swab [24.0%], and cerebrospinal fluid [20.0%]. The high prevalence of MRSA in our setup should not go without serious concern. Implementation of strict aseptic techniques and suitable antimicrobial policy may reduce the spread of MRSA in our environment


Assuntos
Humanos , Staphylococcus aureus , Hospitais , Estudos Transversais , Oxacilina
3.
JPMA-Journal of Pakistan Medical Association. 2003; 53 (10): 445-447
em Inglês | IMEMR | ID: emr-63057

RESUMO

To identify the Leishmania species being responsible for cutaneous leishmaniasis in Multan. Parasites were isolated from clinically and parasitologically confirmed lesions of cutaneous leishmaniasis from 30 patients by fine needle aspiration [FNA]. The bioptical materials were then cultured in Evans Tobie's medium and parasites isolated were identified by isoenzyme electrophoresis technique. Successful Leishmania isolates were obtained from 16 patients. All strains were identified by biochemical techniques as belonging to Leishmania tropica zimodeme MON7 variant PGD. The causative species was identified as Leishmania tropica


Assuntos
Humanos , Masculino , Feminino , Leishmania/isolamento & purificação , Leishmania tropica/isolamento & purificação
4.
Annals of King Edward Medical College. 1999; 5 (1): 78-79
em Inglês | IMEMR | ID: emr-50297

RESUMO

Twenty three patients of psoriasis were treated with thrice weekly PUVA therapy. Complete clearing of the disease was seen in 20/23 patients [87%]. On the average, a patient required 25 exposures, cumulative dose of 137 J/cm2 and 8.5 weeks for clearance. The treatment was discontinued in three patients due to worsening of the disease. After clearing of the disease, 18 patients [90%] received maintenance therapy, which was then tapered off. Only 8 patients relapsed after 6 months of stoppage of the treatment. The most common side effect noted was nausea [30%], followed by pruritus [23%], erythema [13%], headache [7%], Koebner's phenomenon [5%] and blister formation [4%]


Assuntos
Humanos , Masculino , Feminino , Terapia PUVA , Resultado do Tratamento
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