Compensatory ovarian changes, mast cell distribution and luminal structure change follwing unilateral ovariectomy in rats

To follow-up the present study, 40 mature female rats were used. The animals were divided into test, control and sham groups. The rats in the test group were laparotomized and unilateral ovariectomy was done. On the 1st and 2nd met-oestrous after operation, the tissue samples were dissected out and processed for histological study. The intact ovary in the test group underwent a significant [P<0.05] hypertrophy and compensatory changes including double angiogenesis, high cytoplasmic vacuolation in granulose cell types of corpus luteum, compensatory follicular development, decreasing of follicular atresia, approximately double ovulation rate and increase in the width of cortex and medulla. The uterine horn on the intact side was approximately similar to the control and sham, while morphometric analyses showed that the horn on the ovariectomized side had significant decrease in the thickness of different layers. Scattering of endometrial glands on the uterine horn of the intact side was normal, showing a dense basophilic appearance in haematoxyline and eosin staining sections. The horn on the ovariectomized side showed low gland scatterin. Histomorphometric analyses showed no significant differences between the vaginas in the three different groups. Distribution of mast cells [MCs] as essential cells participating in angiogenesis was investigated. In the test group, mast cells were considerably increased in number around the blood vessels in the medulla of the intact ovary and intact uterine horn. Mast cells were very low in number on the horn on the ovariectomized side

Macro anatomical investigations of the cranial cervical ganglion of the sheep

In the present study, eight specimens of sheep [>1 year] of both sexes were dissected to provide a comprehensive description of the weight, allocation and nerve branches of the cranial cervical ganglion. The cranial cervical ganglion was found beneath the mandibular salivary gland. It was located ventromedial to tympanic bulla and ventrally to atlantic fossa. In three out of eight animals it was at the dorsal region of the base of epiglottic cartilage, medial to paramastoid process and medial of the external carotid and occipital arteries. The cranial cervical ganglion was fusiform and reddish in colour. The weight, length, width and thickness [Mean +/- SE] of the cranial cervical ganglion in sheep were 0.12 +/- 0.01 g, 8.52 +/- 0.34 mm, 2.31 +/- 0.03 mm and 2.00 +/- 0.03 mm, respectively. It was found that the branches of the cranial cervical ganglion were internal carotid, external carotid and jugular nerves