RESUMO
Objective: To detect Hemoglobin variants D and S in dried blood spot collected on Guthrie card
Materials and Methods: This study was conducted in Gulf Medical University Ajman on known positive samples for hemoglobin S and hemoglobin D. Known EDTA blood sample was spotted on Guthrie card, the hemolysate of a punched disc collected from blood spot was eluted and the elute examined by Tosoh G7 using HPLC method1
Results: HPLC analysis of the eluate could correctly identify the hemoglobin's subtypes with 100% concordance in detection for hemoglobin D and hemoglobin S
Conclusion: HPLC analyzed the eluate from blood spot on card can correctly detect hemoglobin D and hemoglobin S. Dried blood spot card could be used to screen newborn for presence of hemoglobin S and hemoglobin D