Role of fine needle aspiration cytology in children with peripheral lymphadenopathy

To see the accuracy and role of fine needle aspiration cytology in children with peripheral lym-phadenopathy in our setup. Descriptive study. Department of Pathology Peoples Medical College Nawabshah. January 2002 to December 2004. A total number of 158 children were included in the study having age upto 12 years with history of peripheral lymphadenopathy. Fine needle aspiration cytology was performed with 22 gauge needle attached with 10 ml syringe and without using any local anesthetic. Confirmation of the diagnosis by subsequent surgical biopsy or by a clinical follow up period for at least six months was mandatory. 179 aspirates were obtained from 158 patients. The cytological diagnosis were; 78 [43.6%] reactive, 43 [24.0%] tuberculous lymphadenitis, 27 [15.1%]suppurative inflammation, 15 [8.3%] non Hodgkin's lymphoma, 05 [2.8%] Hodgkin's lymphoma, 03 [1.7%] leukemic infiltration, 02 [1.1%] cytological diagnosis uncertain, 06 [3.4%] material inadequate. 06 cytological diagnosis were false negative and one was false positive. The overall accuracy was 96%. Fine needle aspiration cytology is an initial important diagnostic tool in children having peripheral lymphadenopathy, especially in those rural areas where the surgical facilities are not available. It is a safe, rapid simple and accurate with low morbidity -and only the doubtful cases may go for surgical biopsy

Significance of her-2/neu oncoprotein overexpression in invassive ductal breast cancer

To asses the frequency of HER-2/neu Oncoprotein over expression in invasive ductal breast cancer and correlate it with various prognostic parameters. Cross-sectional study. Department of pathology Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. Seventy two [72] formalin fixed paraffin embedded blocks of diagnosed invasive ductal carcinoma were retrieved and 5 p.m thick sections were cut and stained with H and E for the review of diagnosis and grading. The immunohistochemical staining was done on 4p.m thick sections by using Polyclonal Rabbit anti HER-2 ZYMED USA, and ZYMED 2nd generation LAB-SA immunodetection system, to see the HERVneu over expression. Section containing > 50% of tumor cells exhibiting intense circumferential cell membrane stainingscored as positive. Thirty one [31%] positive over expression was observed. A statistically significant result [P<.05] was found between HER-2/neu over-expression and lymph node status and size of tumor. Oncoprotein HER-2 / neu over expression is directly related to the lymph node status and size of the tumor. These patients may benefit from a more aggressive therapy like Herceptin and can also be used as a prognostic marker in the follow up of these patients

Role of fine needle aspiration cytology in children with peripheral lymphadenopathy

To find out the accuracy and role of fine needle aspiration cytology in children with peripheral lymphadenopathy. Descriptive study. Department of pathology, Peoples Medical College Nawabshah. January 2002 to December 2004. A total number of 158 children were included in the study having age up to 12 years with history of peripheral lymphadenopathy. Fine needle aspiration cytology was performed with 22 gauge needle attached with 10 ml syringe and without using any local anesthetic. Confirmation of the diagnosis by subsequent surgical biopsy or by a clinical follow up period, for at least six months was done. One hundred seventy nine aspirates were obtained from 158 patients. The cytological diagnosis was reactive change in 78 [43.6%], tuberculosis in 43 [24.0%], 27 [15.1%] suppurative inflammation, 15 [8.3%], non Hodgkin's lymphoma, 05 [2.8%] Hodgkin's lymphoma, 03 [1.7%] leukemic infiltrations, 02 [1.1%] cytological diagnoses uncertain and in cases 06 [3.4%] material was inadequate. Six cytological diagnosis were false negative and one was false positive. The overall accuracy was 96%. Fine needle aspiration cytology is an initial important diagnostic tool in children having peripheral lymphadenopathy, especially in those rural areas where the surgical facilities are not available. It is a safe, rapid simple and accurate with low morbidity and only the doubtful cases may go for surgical biopsy

Evaluation of risk factors of incisional hernia

Incisional hernia is an important complication of abdominal surgery. This study was conducted on 52 patients evaluation at Peoples Medical College Hospital NawabShah from January 1996 to December 2000, in which generally accepted risk factors for developing the primary incisional hernia were evaluated. In this study male to female ratio was 1:3, 42 patients had initial surgical procedure in emergency and 10 were operated on elective list. 9 [17.31%] cases were obese, 6 [11.54%] had history of raised intra-abdominal pressure [Chronic cough, Chronic constipation, Prostatism], 34 [65.38%] cases had evidence of wound infection. 47 [90.38%] cases had vertical incisions [Midline 38 [73.01%], Para median 9[17.31%]j and 5 [9.62%] cases had other incisions [Kocher's, Gridiron and Transverse]. Absorbable sutures were used in 39 [75%] cases and non-absorbable [Proline] were found in 13 [25%]