RESUMO
In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2 percent). Four out of the 20 patients with AL (20.0 percent) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for e1a2, while in the BCR-ABL(+)AML patients a b3a2 rearrangement was observed. In conclusion, multiplex-PCR allows rapid, specific and simultaneous detection of different types of BCR-ABL transcripts in CML and ABL-BCR(+)AL. A full correlation was observed when multiplex-PCR was compared with nested PCR.
RESUMO
El uso de tabaco entre la gente joven y los adolescentes es un problema de importancia mayor para la Salud Publica. El habito de fumar comienza siempre en la adolescencia temprana. Se han descripto numerosos factores de riesgos que influyen en la iniciacion del habito de fumar en los mismos. Aunque la prevalencia y algunos factores de riesgo han sido mostrados en trabajos realizados en Buenos Aires, muy poco es lo que se conoce sobre el uso del tabaco en adolescentes en otros sitios de la Republica Argentina...