Mechanism of Zhibai Dihuang decotion in treating postmenopausal osteoporosis / 药学学报

In this study, the ovarian surgery (ovariectomy, OVX) was used to establish the osteoporosis mice model of primary menstruation, in order to evaluate the protective effects and mechanisms of Zhibai Dihuang decotion on postmenopausal osteoporosis (PMOP). The animal experimental protocol has been reviewed and approved by Laboratory Animal Ethics Committee of Jinan University (number: 20210315-03), in compliance with the Institutional Animal Care Guidelines. C57BL/6 mice were divided into five groups, including Sham group, OVX group, low (32 g·kg-1·day-1) and high dose (64 g·kg-1·day-1) of Zhibai Dihuang decotion groups, positive drug group (alendronate, 9.9 mg·kg-1·q3d). After modeling, mice were given medication intervention for 8 weeks, and then femoral and tibial tissues were taken to detect indicators such as bone microstructure, bone resorption, and oxidative stress. The experimental results showed that after Zhibai Dihuang decotion administration, the bone microstructure damage caused by OVX surgery was alleviated, and the relevant parameters bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular number (Tb. N) and connectivity density (Conn. D) both significantly increased. At the same time, the number of TRAP positive osteoclasts decreased significantly, and the levels of proteins and genes related to osteoclast differentiation decreased, indicating that Zhibai Dihuang decoction could inhibit the increased activity of osteoclast caused by OVX. Afterwards, network pharmacology was used to construct the active compound action target network of Zhibai Dihuang decotion, and it was found that the target genes of its active ingredients were closely related to the oxidative stress pathway. Finally, the detection results of oxidative stress levels in bone tissues showed that after treatment with Zhibai Dihuang decotion, the levels of oxidative stress products 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) in bone tissues of mice significantly decreased, while the levels of antioxidant stress substance L-glutathione (GSH) increased. These above results indicated that Zhibai Dihuang decotion can regulate the level of oxidative stress in the body and inhibit osteoclast activity, which played a therapeutic role in PMOP, as well as provided theoretical basis for the prevention and treatment of PMOP with traditional Chinese medicine.

Research advances of phospholipid peroxidation in pathogenesis of coronary heart disease / 中国药理学通报

Coronary heart disease (CHI)) is a kind of coronary atheroselerotie cardiovascular disease that seriously endangers human health and needs to he solved urgently in the world.Epidemiological

Introduction of image-guided stereotactic radiotherapy systems for small animals / 中华放射肿瘤学杂志

The theory and application of small animal radiotherapy models is critical for the research and development of radiobiology and clinical radiotherapy.Considering universality and cost effects,mouse models are widely used to explore the radiobiological mechanisms of cancerous and normal tissues.In recent years,there has been tremendous progress in image-guided stereotactic radiotherapy equipment for small animals,which could simulate the human radiotherapy process.This article introduces stereotactic radiotherapy systems for small animals guided by different imaging modalities,such as cone-beam computed tomography and magnetic resonance imaging,and then reviews small animal fluorescence imaging technology and summarizes the application of different bioluminescence and fluorescence imaging equipment in small animal imaging systems.Finally,we put forward the prospect of optimization direction of radiotherapy equipment for small animals in future.