Factores de Riesgo relacionados al Síndrome de Estrés Medial Tibial en Corredores Competitivos: Una Revisión Sistemática / Risk Factors related to Medial Tibial Stress Syndrome in Competitive Runners: A Systematic Review

Introducción. El síndrome de estrés tibial medial (SEMT) tiene una alta prevalencia entre corredores y genera un negativo impacto funcional. Un gran número de factores de riesgo se asocian con el desarrollo de SEMT, sin embargo, no existe claridad de cuales factores desencadenan la lesión en esta población. Objetivo. Identificar los factores de riesgo relacionados al SEMT en corredores sintetizando la información en una revisión sistemática. Métodos. Se realizó una búsqueda de estudios de cohorte y, casos y controles, en idioma inglés y español, en distintas bases de datos tales como Pubmed, EMBASE, EBSCO, SPORTDiscus, Scopus y Web of Science. Se incluyeron estudios con población de corredores de cross-country y track and field. Se analizaron factores de riesgo asociados a SEMT. Además, Se extrajeron datos como diseño y duración del estudio, definición del SEMT utilizada, características de la población y método diagnóstico. Se evaluó la calidad metodológica con el "NIH Quality Assessment Tool". Resultados. Cinco estudios y 43 factores de riesgo fueron analizados. 18 factores de riesgo presentaron una asociación significativa con SEMT. Los estudios tuvieron entre 64,3-85,7% de calidad metodológica. Los factores de riesgo más analizados fueron sexo, caída del navicular, índice de masa corporal, talla, peso, rango de dorsiflexión de tobillo y rotación de cadera en el plano transverso. Conclusión. Nuestra revisión no permitió determinar un factor de riesgo de SEMT, ya que los resultados entre los estudios fueron contradictorios o solo un estudio mostraba resultados significativos.

Background. Medial tibial stress syndrome (MTSS) has a high prevalence among runners and creates a major functional impact. A large number of risk factors are associated with the development of MTSS, which are not conclusive in this population. Objective: To identify the risk factors associated with MTSS in runners summarizing the gathered data through a systematic review. Methods. A search for cohort and case-control studies retrieved from different databases was conducted in English and Spanish. Studies with cross-country, track, and field runners were covered, in which risk factors associated with MTSS will be analyzed. The extracted data included: the design and duration of the study, the definition of MTSS currently used, the characteristics of the population, the analyzed risk factors, and the diagnostic method. The methodological quality was screened through the use of the "NIH Quality Assessment Tool". Results. Five studies and 43 risk factors were analyzed. Eighteen risk factors had a significant association with MTSS. The studies had a methodological quality of 64.3 -85.7%. The most analyzed risk factors were gender, navicular drop, body mass index, height, weight, range of ankle dorsiflexion and hip rotation in the transverse plane. Conclusion. Our review did not allow us to determine a risk factor for SEMT, since the results between the studies were contradictory or only one study showed significant results.

In vitro and in vivo antifungal activity, liver profile test, and mutagenic activity of five plants used in traditional Mexican medicine

Berberis hartwegii Benth., Berberidaceae, Hamelia patens Jacq., Rubiaceae, Dendropanax arboreus (L.) Decne & Planch., Araliaceae, Erythrina herbacea L., Fabaceae, and Zanthoxylum caribaeum Lam., Rutaceae, acetone extracts were selected on the basis of their use in traditional Mexican medicine to treat scabies or skin diseases. Anti-dermatophyte activity in vitro was evaluated using the agar dilution assay, and the therapeutic efficacy of B. hartwegii and Z. caribaeum were tested against experimental tinea pedis. The infected animals were treated intragastrically daily for seven days with 2.5 and 5 mg/kg of acetone extracts. The acetone extract of H. patens exhibited 100% growth inhibition against T. mentagrophytes and E. floccosum at 100.0 and 50.0 µg/ml, respectively, and B. hartwegii inhibited growth of M. canis and T. mentagrophytes at 100.0 µg/ml. Effective treatments with 2.5 mg/kg of Z. caribaeum and B. hartwegii extract were comparable with 1 mg/kg of clotrimazole in mice. Liver profile tests and histological analyses did not exhibit any signs of toxicity and the Ames test indicated that both extracts were safe when evaluated in strains TA98, TA100 and TA102. Our results suggest the potential for the future development of new antifungal drugs from B. hartwegii or Z. caribaeum.

Acute and subacute toxicity (28 days) of a mixture of ursolic acid and oleanolic acid obtained from Bouvardia ternifolia in mice / Toxicidad aguda y subaguda (28 días) en ratones, de la mezcla de ácido ursólico y ácido oleanólico obtenida de Bouvardia ternifolia

Ursolic acid (UA) and oleanolic acid (OA) are triterpenes that are found in a large number of medicinal plants, one of which is the species Bouvardia ternifolia. These compounds have been shown to have around 120 types of biological activity, especially the hepatoprotective, anti-inflammatory and antimycobacterial effects. Despite having a high therapeutic potential, not much information concerning their toxicity is available. This article describes the results of acute and subacute (28 days) toxicity evaluations in Balb/c mice (both sexes) treated with the mixture of UA/OA obtained from B. ternifolia at doses of 6.5 and 13 mg/kg. The LD50 was >300 mg/kg. During the subacute administration, there was no death of animals and no changes were observed in the growth or weight of the different organs when compared to the control groups. Studies of blood chemistry and blood count showed normal levels in all parameters evaluated. The histopathology of major organs showed no changes or abnormalities. The mixture UA/OA is indeed safe when administered subcutaneously as a single dose of 300 mg/kg or in repeated doses of 13 mg/kg during 28 days.

Los ácidos ursólico (UA) y oleanólico (OA) son triterpenos que se encuentran distribuidos en un gran número de plantas medicinales, una de ellas es la especie Bouvardia ternifolia. Estos compuestos han mostrado alrededor de 120 actividades biológicas, destacando los efectos hepatoprotector, antiinflamatorio y antimicobacteriano. A pesar de ser compuestos con un alto potencial terapéutico, no se han documentado muchos datos acerca de su toxicidad. En este artículo se describen los resultados de la evaluación de toxicidad aguda y subaguda (28 días) en ratones Balb/c de ambos sexos, tratados con la mezcla de AU/AO obtenida de B. ternifolia a dosis de 6.5 y 13 mg/kg. La DL50 fue > 300 mg/kg. Durante la administración subaguda, no hubo muerte de animales, tampoco se observaron alteraciones en su crecimiento ni alteraciones en el peso de los diferentes órganos. Los estudios de biometría hemática y química sanguínea mostraron niveles normales en todos los parámetros evaluados. Los análisis histopatológicos de los principales órganos no presentaron cambios o anormalidades. La mezcla UA/OA es prácticamente inocua cuando se administra subcutáneamente en dosis única de 300 mg/kg y 13 mg/kg en dosis repetida (28 días).

Differentiation of oxyntic cells and cell-matrix interactions during avian gastric gland morphogenesis

The relation between the expression of the oxyntic cell phenotype and the modifications of the extracellular matrix during development of the gastric glands, was studied in 10 to 21 day-old chick embryos. Cytodifferentiation of the oxyntic cells was established by ultrastructural methods, while the expression of pepsinogen, mitochondrial enzyme markers and apical secretory membranes was determined by histochemical and biochemical procedures. Results show that the morphogenesis of the glandular lobules occurs between days 8 and 15 of gestation. Later on, the lobules enlarge but maintain their basic morphology. Until day 13, the developing glands consist of primary tubes lined by a stratified columnar epithelium. The apical poles of the cells that contact the lumen show cytoplasmic processes, and Mg-ATPase activity and F-actin are concentrated at the apical cell borders. From day 13 on, the cells of the simple epithelium that lines secondary tubules budding from the primary tube, show all the features that define differentiated oxyntic cells. The synthesis of glycosaminoglycans during glandular morphogenesis was studied measuring the incorporation of radioactive sulfate into developing chick embryo proventriculi. An important increase in isotope incorporation was found between days 13 and 18 of development. Histochemical localization of these macromolecules shows that glycosaminoglycans are closely associated with the developing glandular lobules. Variations in the structure of epithelial cells undergoing morphogenesis and in the composition of the extracellular matrix are synchronous, suggesting that interactions between them may be significant in terms of the establishment and maintenance of the adult gastric gland phenotype