RESUMO
<p><b>OBJECTIVE</b>To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system.</p><p><b>METHOD</b>Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed.</p><p><b>RESULT</b>The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence.</p><p><b>CONCLUSION</b>The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense</p>
Assuntos
Análise de Variância , Primers do DNA , DNA de Plantas , Genética , Dendrobium , Genética , Variação Genética , Germinação , Genética , Plantas Medicinais , Genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Fatores de Tempo , Técnicas de Cultura de TecidosRESUMO
This paper summarizes the studies on improving crop nutritional quality including protein, saccharide and lipid through gene engineering in recent 10 years. Special emphasis is laid upon the improvement of protein contains and amino acid components. The food safety caused probably by gene engineering and some ways to solve the problem are introduced briefly.