RESUMO
<p><b>AIM</b>To study the chemical constituents of Marsdenia tenacissima (Roxb.) Wight et Arn.</p><p><b>METHODS</b>To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis.</p><p><b>RESULTS</b>Two compounds were isolated from Marsdenia tenacissima and their stuctures were determined as tenacissosides J (I) and tenacissosides K (II).</p><p><b>CONCLUSION</b>Compounds I and II are new C21 steroidal glycosides.</p>
Assuntos
Glicosídeos , Química , Marsdenia , Química , Conformação Molecular , Estrutura Molecular , Caules de Planta , Química , Plantas Medicinais , Química , Esteroides , QuímicaRESUMO
<p><b>AIM</b>To investigate the chemical constituents of tetraploidy Banlangen (Isatis indigotica Fort.).</p><p><b>METHODS</b>Compounds were separated by chromatography on silica gel. Their structures were determined by spectral analysis and chemical evidence.</p><p><b>RESULTS</b>Three compounds were isolated. Their structures were identified as (E)-2-[(3'-indole) cyanomethylene]-3-indolinone (I), 2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxy-phenyl)-methyl]-3- hydroxymethyl-tetrahydro-furan (II) and 2-methoxy-4-[tetrahydro-4-[(4-hydroxy-3-methoxy-phenyl)-methyl]-3- hydroxymethyl-2-furanyl] phenyl-1-O-beta-D-glucopyranoside (III).</p><p><b>CONCLUSION</b>Compound I is a new compound.</p>
Assuntos
Indóis , Química , Isatis , Química , Genética , Estrutura Molecular , Raízes de Plantas , Química , Plantas Medicinais , Química , Genética , PoliploidiaRESUMO
Objective: To establish capillary zone electrophoresis method for determination of sildenafil citrate (Viagra) content in its troche. Methods： Using tetrandrine as internal standard(IS), the electrophoretic separation was achieved with 25 mmol/L borate (pH=7.89) running buffer. And a voltage of 14 kV was applied to the 40 cm×75 μm(i.d) capillary. The analytes were introduced into capillary by siphon (1 s) and determined with on-column monitoring at 214 nm. Results：The determination could be completed within 4 min and the minimum concentration of detection was 5 μg/ml.The analytical results of sildenafil citrate samples demonstrated a good linear relationship within the range of 24-480 μg/ml.The relative standard deviations (RSD) of intra-day was less than 1.58% and that of inter-day was less than 2.46%.The present recoveries were between 95%-105％. Conclusion：The CZE method is accurate, simple, rapid and reliable, so it can be applied to the determination of sildenafil citrate content.
RESUMO
Objective: To establish capillary zone electrophoresis method for determination of sildenafil citrate (Viagra) content in its troche. Methods： Using tetrandrine as internal standard(IS), the electrophoretic separation was achieved with 25 mmol/L borate (pH=7.89) running buffer. And a voltage of 14 kV was applied to the 40 cm×75 μm(i.d) capillary. The analytes were introduced into capillary by siphon (1 s) and determined with on-column monitoring at 214 nm. Results：The determination could be completed within 4 min and the minimum concentration of detection was 5 μg/ml.The analytical results of sildenafil citrate samples demonstrated a good linear relationship within the range of 24-480 μg/ml.The relative standard deviations (RSD) of intra-day was less than 1.58% and that of inter-day was less than 2.46%.The present recoveries were between 95%-105％. Conclusion：The CZE method is accurate, simple, rapid and reliable, so it can be applied to the determination of sildenafil citrate content.