RESUMO
BACKGROUND: Lonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films. METHODS: For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiO2ntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells' genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton's Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay. RESULTS: The highest differentiation capacity of MOMCs was observed on PCL/SiO2ntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation. CONCLUSION: To conclude, the differentiation of MOMCs was selectively guided by incorporating SiO2ntbs or SrHAnrds into a polymeric matrix, for the first time.
Assuntos
Autoenxertos , Beleza , Linhagem Celular , Linhagem da Célula , Durapatita , Endotélio , Citometria de Fluxo , Células Endoteliais da Veia Umbilical Humana , Células-Tronco Mesenquimais , Métodos , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Nanocompostos , Nanotubos , Osteoblastos , Osteogênese , Polímeros , Reação em Cadeia da Polimerase em Tempo Real , Medicina Regenerativa , Dióxido de Silício , Estrôncio , Geleia de WhartonRESUMO
Comparison of prooxidant-antioxidant balance [PAB] assay with crocin assay. Twenty eight serum samples were chosen, PAB and the total antioxidant capacity were measured by PAB assay and crocin, respectively, and the correlation of both assays, along with their correlation with other clinical and biochemical parameters, were determined. A significant negative correlation was established between PAB assay and crocin assay. Also a significant negative correlation was established between PAB and uric acid and creatinine. The results showed that by increasing the total antioxidant capacity, which is showed by crocin, the PAB shifts in favor of antioxidants, which is showed by PAB assay. Now, it could be considered that the PAB, along with other risk factors, might help in the prediction of the risk for cardiovascular events; and further research could clarify whether by application of PAB assay and appropriate interventions for correcting oxidative stress, progression of the cardiovascular disease could be reduced