RESUMO
Conjunctivitis is one of the most prevalent ophthalmic diseases. The most feasible treatment of that is using antibiotics. According to arising bacterial resistance to usual antibiotics and their side effects, and using of tea as a healing for ophthalmic diseases base on the traditional beliefs, this study was done to evaluate the antibacterial effect of tea on conjunctivitis diseases. In this experimental study, patients suffering from conjunctivitis were sampled from the ophthalmology clinic in Shahrekord, Iran. Samples were transferred to the microbiology lab and were subjected to differential culture media and diagnostic tests. Ten samples from each group [totally 30] were isolated from each of the three bacteria including S. aureus, S. epidermidis and S. pneumonia. These strains were separately tested using the pour plate technique with different dilutions of black tea extract. In addition, antibiograms were performed using standard antibiotic disks including vancomycin, Chloramphenicol, Oxacillin, Cefazolin and Ciprofloxacin. Data were analyzed using Chi square, t test and ANOVA. Findings of this study showed that tea extract had a dose dependant inhibatory effects on bacterial growth [P<0.05]. This antibacterial effect exists in densities of 50. This effect had a rise in shifting from 50 to 100 mg/ml [P<0.05]. According to this study's findings and comparison with similar previous studies, we might suggest tea extract as an antibacterial drug in conjunctivitis
RESUMO
Bacterial resistance to antibiotics is a main problem in the treatment of infectious diseases. Thus, searching for alternative drug is essential in Iran and particularly Chaharmahal va bakhtiari province. People use medicinal smokes such as donkey dung and Peganum harmala seed smokes for treatment of infectious diseases. Therefore, this study was aimed to evaluate the antimicrobial property of donkey dung and Peganum harmala seed smokes on Staphylococcus aureus and Pseudomonas aeroginosa. In this interventional and laboratory study, groups of Peganum harmala seed smoke and donkey dung were considered as case groups and antibiotic disks as positive control group. Pseudomonas aeroginosa and Staphylococcus aureus were cultured in suitable medium [Blood Agar, EMB and Mueller-Hinton agar]. Antibiogram blank disks were fumigated separately with Peganum harmala seed and female donkey dung smoke then placed on microbial plate with sterile methods. Following 48 hours incubation at 37°C, the zone of growth inhibition evaluated by measuring the zone around the disks. Fumigation process was done in special chest that designed for this research. We repeated fumigation each 20 minutes for 24 times. Data about measuring the zone of growth inhibition were analyzed by using and mean statistic exam. Staphylococcus aureus was sensitive to Peganum harmala seed, and fdonkey dung smokes and Pseudomonas aeroginosa was sensitive to female donkey dung smoke. Staphylococcus aureus was resistant to cloxacilllin and Pseudomonas aeroginosa was sensitive only to erythromycin and ciprofloxacin. The increasing time of fumigation in sensitive cases enhanced antimicrobial effects and the zone of growth inhibition. Antimicrobial effects of donkey dung smokes on resistance pathogens such as Pseudomonas aeroginosa, Staphylococcus aureus revealed the necessity of performing expanded research about composition and property of this smoke