RESUMO
Background: dermatomycoses are a major public health problem. Tinea cruris is a form of Dermatomycoses. Tinea cruris is a common infection in the groin area, genitals, pubic area, perineum and perianal skin.The purpose of thisstudywas to investigate the cutaneous fungal infections among students in a school dormitory
Materials and Methods: the study was performed on 110 male students dormitory aged between 18 to 28 years.From the specimens obtained; direct preparations were made using %10 potassium hydroxide and cultured on sabouraud dextrose agar containing 0.05 mg/ml chloramphenicoland 0.5 mg/ml cycloheximide [Scc]. Identification of fungi was performed according to morphologic and microscopic growth of the colonies and using slide culture method
Results: 17 people out of 110 individuals were diagnosed with cutaneous fungal infections. Six people were diagnosed with dermatophytosis. All cases of dermatophytosis were Tinea cruris. Epidermophyton floccosum, the anthropophilic species, was only isolated dermatophyte.The prevalence of Tinea cruris was 5.5% and the Prevalence of Pityriasis versicolor was 4.5%
Conclusion: the most important source of transmission of anthropophilic dermatophyte species was by men to men.The results of this study is similar to most other studies in Iran and other countries. In addition to treatment, other necessary steps should be applied in order to prevent infection and reduce the risk of pathogens
RESUMO
OBJECTIVE@#To clone the virB12 gene in pET28a expression vector for production of recombinant protein to be used as antigenic component for future serological test development.@*METHODS@#Brucella melitensis (B. melitensis) 16M strain was cultured and bacterial DNA was extracted by Bioneer AccuPrep® Genomic DNA Extraction Kit. Oligonucleotide primer pair was designed based on Brucella virB12 gene sequence with BamHI and HindIII restriction site at 5' end of the forward and reverse primers, respectively. DNA amplification was performed using PrimSTAR® HS DNA polymerase and the PCR product was purified by DNA AccuPrep®Gel Purification Kit. Purified DNA was cloned into pJET1.2 cloning vector. VirB12 gene fragment was excised from pJET1.2 using BamHI/HindIII and subsequently subcloned into pET28a (+).@*RESULTS@#Brucella virB12 gene was successfully cloned in pJET1.2 and then in pET28a (+) plasmids. PCR and restriction enzyme digestion confirms the procedure.@*CONCLUSION@#We cloned and expressed the Brucella virB12 gene which could be used as antigenic component for specific serological assay development.
Assuntos
Antígenos de Bactérias , Genética , Proteínas de Bactérias , Genética , Brucella melitensis , Genética , Clonagem Molecular , Métodos , DNA Bacteriano , Genética , Vetores Genéticos , Genética , Técnicas de Amplificação de Ácido Nucleico , Reação em Cadeia da Polimerase , Métodos , Proteínas Recombinantes , Genética , Testes Sorológicos , MétodosRESUMO
Diarrhea is one of pediatric infectious diseases, which is the most frequent cases of child mortality. Diarrhea is a health problem in Iran; nearly 70 thousand children were died from diarrhea. Several bacterial agents can cause diarrhea, one of them is Campylobacter jejuni, which is less considered. The aim of this study is to determine the frequency of Campylobacter jejuni in Semnan diarrheic children. Stool samples were collected from diarrheic children who refer to Amiralmomenin hospital, Shafa hospital and hygiene centers of Semnan and these children did not receive any antibiotic treatment at the beginning of the study. A swab was inoculated in Stuart medium and transferred to clinical laboratory of Semnan hygiene center. The swab was inoculated on Preston blood free Campylobacter agar and incubated at 42°C for 48 hours. In order to bacterial identification, gram stain, catalase, oxidase, lack of H2S production in TSI, susceptibility to Nalidixic acid, resistance to Cephalotin and Hyporate hydrolysis were used. Antibiotic susceptibility test was performed by Kirby-Bauer method. From 276 stool specimens [38.5% females and 61.5% males], Campylobacter jejuni was isolated from 27 cases [9.8%; 44.4% females and 55.6% males]. The most susceptibility was seen for Erytromycin [92.6%], and the most resistance was observed for Cotrimoxazole [44.4%]. In this study, the prevalence of Campylobacter jejuni was more than other studies. Therefore, notification to Campylobacter jejuni in patient treatment and bacterial transmission prevention is necessary in this area