Natural Indicator as a substitute to Synthetic indicator-A Developmental Approach.

Synthetic indicators had perpetually been an alternative choice for all types of titration since long time. However price had always been a tangle. Therefore development of an indicator from natural source i.e. from the flower extracts had been the main aim of this present research work. The current work complies data regarding the comparison of Natural indicator and synthetic indicator and whether or not it may be substituted from Synthetic indicator.

Drug delivery through osmotic systems – An overview.

The immediate release conventional dosage form lack in the efficiency of controlling the proper plasma drug concentration. This results in the development of various controlled drug delivery system. Among which the Pulsatile drug delivery systems (PDDS)/ osmotic drug delivery system (ODDS) are gaining importance as these systems deliver the drug at specific time as per the path physiological need of the disease, resulting in improved patient therapeutic efficacy and compliance. They work on the principle of osmotic pressure for controlling the delivery of the drug. The release of the drug is independent of physiological factors of the GIT to a large extent. This review highlights’ the theoretical concept of drug delivery, history, types of oral osmotic drug delivery systems, factors affecting the drug delivery system, advantages and disadvantages of this delivery systems ,theoretical aspects, applications, marketed status and last but not the least the recent development.

Studies on effect of variables by response surface methodology for losartan microspheres.

Microspheres of Losartan potassium were formulated using combination of Ethyl cellulose and Acycoat L30D polymer by solvent evaporation method. A 32 factorial design was used to elucidate the effect of variables viz. the amount of drug and the amount of polymer. Discrete spherical microspheres in the range of 40-50 μm were produced with the encapsulation efficiency of more than 80 %. Polynomial equations and response surface plots were generated for all dependent variables. It was observed that both the factors had significant influence on all dependent variables studied. It was observed that as the amount of polymer increases, the rate of release decreases. So t50 increases and the amount of drug released in 2 h (X120) decreases. Drug polymer interaction study was absent as evidenced by HPLC and FT-IR studies.

Wheat (Triticum aestivum) peptide (s) mimic gibberellin action and regulate stomatal opening.

Wheat peptides (0.5 to 3 KDa Mr) mimick hormonal activity like that of gibberellins and forced open dark closed stomata. The deionized amphoteric peptides solution after passing through cation and anion exchanger resins was run through Amicon’s ultrafilters, 10, 3 and 0.5 kDa (Mr) cut off system. The 3 to 0.5 kDa fraction passed through sephadex LH-20 column and collected in 140 tubes (5 ml in each tube). The two fractions F 9 (91-100 tubes) and F 12 (121-130) were found much active on stomatal opening and -amylase activity, respectively and were ninhydrin positive. Capillary electrophoresis of F 9 fraction yielded several peptides ranging 1600 to 2200 (Mr) and F 12 fraction showed 1800 – 2800(Mr). Both the fractions were totally hydrolysed for amino acid analysis by HPLC. Most of the amino acids were present except cystein in both the fractions. The F 9 fraction, (peptide present in 10 μg fresh wt tissue per ml) induced the dark grown closed stomata to open upto 70%. In F 12 fraction, (peptide present in 10 μg fresh wt equivalent tissue per ml) showed -amylase induction which was much higher than GA3 (10-9 M). The peptide might be present in membrane and bound with GA that activated -amylase m-RNA synthesis. The peptide might act directly on -amylase gene.