Rhodolirium andicola: a new renewable source of alkaloids with acetylcholinesterase inhibitory activity, a study from nature to molecular docking

ABSTRACT Acetylcholinesterase is an important target for control of neurodegenerative diseases causing cholinergic signaling deficit. Traditionally, galanthamine has been used as an Amaryllidaceae-derived acetylcholinesterase inhibitor, although new Amaryllidaceae plants could serve as source for better acetylcholinesterase inhibitors. Therefore, the objective of this study was to characterize the alkaloid composition from bulbs of Rhodolirium andicola (Poepp.) Traub, a native Chilean Amaryllidaceae specie, and assess their inhibitory activity on acetylcholinesterase by in vitro and in silico methodologies. Alkaloidal extracts from R. andicola exhibited an inhibitory activity with IC50 values between 11.25 ± 0.04 and 57.78 ± 1.92 µg/ml that included isolated alkaloid, galanthamine (2.3 ± 0.18 µg/ml), Additionally, 12 alkaloids were detected using gas chromatography-mass spectrometry and identified by comparing their mass fragmentation patterns with literature and database NIST vs.2.0. To better understand the bioactivity of isolated compounds and alkaloidal extracts against acetylcholinesterase, a molecular docking approach was performed. Results suggested that alkaloids such as lycoramine, norpluvine diacetate and 6α-deoxy-tazettine expand the list of potential acetylcholinesterase inhibitors to not only galanthamine. The role of R. andicola as a source for acetylcholinesterase inhibitors is further discussed in this study.

Racionalización del drenaje en la colecistectomía / Rationalization of drainage in cholecystectomy

Se analiza en forma prospectiva y randomizada, la evolución postoperatoria de 138 pacientes colecistectomizados con y sin drenaje. Fueron colecistectomizados sin drenaje 79 y con drenaje 59 pacientes. La morbilidad para el grupo sin drenaje fue de 7,6% y para el grupo con drenaje de 13,6%, cifra que no alcanzó significación estadística. La estada postoperatoria promedio fue de 3,98 días para el grupo con drenaje y de 3,65 para el grupo sin drenaje. Respecto a la convalescencia, esta fue significativamente más confortable para los pacientes sin drenaje (14% contra 3,4%). Se discuten las bases para racionalizar el uso del drenaje en ésta cirugía