RESUMO
ABSTRACT Bioflavonoid-containing diets have been reported to be beneficial in diabetes. In the current study, the effect of Biochanin A (BCA) on blood glucose, antioxidant enzyme activities and oxidative stress markers in diabetic rats were investigated. 30 male Wistar rats were divided into five groups. Two of them were selected as control; group1: control (receiving 0.5%DMSO), and group2: Control+BCA (receiving 10 mg/kg.bw BCA). Diabetes was induced in other rats with injection of (55 mg/kg.bw) streptozotocin; group3: diabetic control (receiving 0.5%DMSO), groups 4 and 5 were treated with 10 and 15 mg/kg.bw BCA respectively. After 6 weeks the following results were obtained. Fasting blood glucose (FBG), Triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C) and malondialdehyde (MDA) levels significantly increased and body weight, high density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD) and catalase (CAT) activity and total antioxidant status (TAS) significantly decreased in diabetic rats as compared to control rats. Oral administration of BCA in 10 and 15 mg/kg.bw, FBG, TG, TC, LDL-C, VLDL-C were decreased significantly in all treated rats. MDA was decreased in all treated rats but it was significant just in 15 mg/kg.bw BCA. HDL, CAT, SOD, and TAS were significantly increased in treated group with 15 mg/kg.bw. The obtained results indicated hypoglycemic and hypolipidemic effect of BCA. Also BCA reduced oxidative stress in diabetic rats.
RESUMO
Previous studies suggested a significant relationship between alpha- amylase and caries formation. This study was implemented in order to investigate the interrelation between level of salivary and serum alpha- amylase, and dental caries. Methods: In this cross-sectional study, un-stimulated whole saliva and serum samples were collected from 118 high school students who were divided into four groups: caries free females (N = 28), caries active females (N = 35), caries free males (N = 28) and caries active males (N =27). Mean levels of salivary and serum alpha-amylase was evaluated by spectrophotometric methods to assayenzyme kinetics. Data were analyzed using studentst-tests and chi-square tests. Results: Salivary alpha amylase was significantly higher in caries active groups as compared to caries free groups (P = 0.002). The mean salivary alpha -amylase was significantly higherin caries active males (P = 0.002). Also, serum alpha-amylase was significantly higher in caries active groups as compared to caries free groups (P = 0.001). In bothsexes the mean serum alpha- amylase levels were statistically significant higher in caries active compared to caries free groups (P = 0.02 and 0.01 respectively). Conclusion: A significant association was found between salivary and serum alpha- amylase and dental caries in adolescents. More research to demonstrate there al relation between alpha amylase and dental caries is recommended...
Objetivo: Alguns estudos anteriores sugeriram uma relação significativa entre a alfa-amilase e a formação de cárie. Este estudo foi realizado com o objetivo de investigar a interrelação entre o nível de alfa-amilase na saliva e no soro e a cárie. Material e Métodos: Neste estudo transversal, amostras de saliva não-estimulada e soro foram coletadas de 118 estudantes do ensino médio que foram divididos em quatro grupos: mulheres livres de cárie (n = 28), mulheres com cárie ativa (n = 35), homens livres de cárie (n = 28) e homens com cárie ativa (n = 27). O nível de alfa-amilase da saliva e soro foi avaliado por espectrofotometria para ensaios de cinética enzimática. Os dados foram analisados pelos testes te Qui-quadrado. Resultados: A alfa-amilase salivar foi significativamente maior nos grupos com cárie ativa em comparação com os grupos livres de cárie (p = 0,002). A alfa amilase salivar foi significativamente maior nos homens com cáries ativa (p = 0,002). Além disso, a alfa amilase sérica foi significativamente maior nos grupos de cárie ativa, em comparação com os grupos livres de cárie(p = 0,001). Em ambos os sexos a média da alfa-amilase sérica foi estatisticamente superior nos grupos de cárie ativa, em comparação com livres de cárie (p = 0,02 e 0,01, respectivamente). Conclusão: Foi encontrada uma associação significativa entre a alfa-amilase salivar e sérica em indivíduos com a cárie dentária na adolescência. São recomendadas mais investigações para demonstrar real relação entre os níveis de alfa-amilase e cárie dentária...