Loss of BRCA associated protein 1 expression in malignant mesothelioma and its diagnostic application / 中华病理学杂志

Objective@#To investigate the expression of BRCA-associated protein 1 (BAP1) in malignant mesothelioma, non-small cell lung cancer and carcinosarcoma, and its application in the differential diagnosis.@*Methods@#Twenty-two cases of malignant mesothelioma including 17 epithelioid type, 2 sarcomatoid type and 3 biphasic type were collected.As the study control, 80 non-small cell lung cancers infringement pleural membrane(including 40 lung adenocarcinomas and 40 lung squamous cell carcinomas) and 15 carcinosarcomas were included. BAP1 expression was detected using immunohistochemical method. A differential diagnosis antibody panel, including calretinin, WT1, CK5/6, D2-40, CAM5.2, CEA, TTF1, Napsin A, p63 and p40 was tested in all cases.@*Results@#All 80 cases of non-small cell lung cancer and 15 cases of carcinosarcoma were BAP1 positive. In contrast, 64% (14/22) of malignant mesotheliomas lost BAP1 expression (P<0.01). Addition of BAP1 to the mesothelioma marker panel, the diagnostic accuracy of malignant mesothelioma was enhanced to 93%. Focal expression of BAP1 in tumors suggested multiclonal evolution of mesothelioma.@*Conclusions@#Loss of BAP1 expression helps to confirm the diagnosis of malignant mesothelioma whereas all non-small cell lung cancer expresses BAP1. It is therefore recommended that BAP1 can be used in conjunction with other immunohistochemical markers to improve the diagnostic accuracy of malignant mesothelioma.

Development of simulation phantom for CT radiation dose measurement / 医疗卫生装备

Objective To develop a simulation phantom for clinical detection of radiation intensity at the organs or tissue vulnerable to X-ray. Methods The organs or tissue vulnerable to X-ray radiation were explored to determine the region of interest of the radiation to the phantom. A mathematical model was established, and the technical characteristics of the equivalent materials of different tissues were studied. The existing ion chamber probes were compared on the technical performances, and the probe was selected with considerations on comprehensive factors. The phantom was gifted with two sizes of adult and child and designed based on the positions of sensitive organs. Practical trials were carried out to test the performances of the phantom. Results The phantom could be used to measure the radiation dose which was equivalent to the value of multiple human organs, and the desired requirements were fulfilled. Conclusion The phantom gains advantages in simulation fidelity, withdrawal of the probe and etc, and thus is worthy promoting practically.

Field X-ray vehicle frame motion control simulation based on Proteus / 医疗卫生装备

Objective To develop a field X-ray vehicle frame motion control simulation circuit based on Proteus.Methods Corresponding component models of Proteus were selected to construct the circuit according to the circuit block diagram.SCM control program was developed and debugged.The frame motion control simulation was executed through dynamic simulation of motor running,simulation results analysis with different parameters and verification of the circuit efficacy.Resnlts DC motor positive and negative rotation controlled by the button was realized,which solved the problems of X-ray vehicle frame motion.Conclusion Proteus can be used to implement system simulation of analog circuit,digital circuit,SCM and peripheral circuit as well as to construct virtual circuit lab.The equipment maintainer enhances medical equipment maintenance based on circuit simulation,circuit model analysis,failure simulation and failure position determination.

Field X-ray vehicle frame motion control simulation based on Proteus / 医疗卫生装备

Objective To develop a field X-ray vehicle frame motion control simulation circuit based on Proteus.Methods Corresponding component models of Proteus were selected to construct the circuit according to the circuit block diagram.SCM control program was developed and debugged.The frame motion control simulation was executed through dynamic simulation of motor running,simulation results analysis with different parameters and verification of the circuit efficacy.Resnlts DC motor positive and negative rotation controlled by the button was realized,which solved the problems of X-ray vehicle frame motion.Conclusion Proteus can be used to implement system simulation of analog circuit,digital circuit,SCM and peripheral circuit as well as to construct virtual circuit lab.The equipment maintainer enhances medical equipment maintenance based on circuit simulation,circuit model analysis,failure simulation and failure position determination.

Roles of ambulatory blood pressure monitoring in the prediction of outcome in patients w ith acute ischemic stroke / 国际脑血管病杂志

Inrecentyears,somecardiovascularguidelineshavegradualyintroducedambulatory blood pressure monitoring ( ABPM ) as an indispensable means for better diagnosis and management of hypertensive patients. ABPM can more comprehensively provide 3 types of information that has potential clinical value:the assessment of real or mean blood pressure level, the diurnal rhythm of blood pressure, and the blood pressure variability. This article review s the roles of ABPM in the outcome assessment in patients w ith acute ischemic stroke.

Influence of sodium citrate concentration in TISAB on detection of water fluoride / 中国地方病学杂志

Objective To observe the influence of sodium citrate concentrations in TISAB on detection of water fluoride,and to explore the feasibility of 0.10 mol/L sodium citrate buffer system in detection of water fluoride.Methods Under pH 5.0 to 5.5,a series of fluoride standards of 0.2,0.5,1.0,2.0 and 5.0 mg/L were measured when sodium citrate concentration was 0.01,0.10,and 1.00 mol/L in the TISAB system.The results of water fluoride measurement were compared,recovery calculated and regression equation of the standard curve was set up.The feasibility of 0.10 mol/L sodium citrate in the TISAB system to detect water fluoride was tested,including interference test,accuracy,confidence limits,as well as precision test.Water fluoride was determined by fluoride ion selective electrode according to the Standard Test Methods for Drinking Water (GB/T 5750.5-2006).Results When the sodium citrate concentrations in the TISAB system were 0.01,0.10 and 1.00 mol/L,and the concentrations of fluoride in the sample were 0.2,0.5,1.0,2.0 and 5.0 mg/L,recoveries of water fluoride were 96.0％,103.0％,179.5％; 80.6％,97.8％,132.2％; 73.3％,97.0％,103.0％; 70.0％,100.0％,87.5％; and 66.4％,102.0％,65.4％.The equation of linear regression was y =lg-1(226.4-E/47.4),y =lg-1(226.4-E/53.4) and y =lg-1(208.1-E/36.9) ; the correlation coefficient(r) were 0.9993,0.9999 and 0.9993.The minimum detectable concentration and limit of quantitation was 0.023 and 0.072 mg/L when sodium citrate was 0.10 mol/L in the TISAB system.Aluminum(Al3+,100 μg),ferrum(Fe3+,800 μg),calcium(Ca2+,1200 μg),Al3+(50 μg) + Fe3+ (800 μg),Al3+(50 μg) + Ca2+(1200 μg) can be masked when sodium citrate was 0.10 mol/L in the TISAB system.The total average recoveries confidence limit R/d was 0.99.The total standard deviation of standard solution,water sample and spiked water sample was less than 5％ of their respective mean concentration.Conclusions High concentration of sodium citrate buffer system has a significant influence on the detection sensitivity and limit of quantification of fluoride ion in water.The capacity of anti-interference of 0.10 mol/L sodium citrate in the TISAB is strong.The confidence limits of precision and accuracy meet the requirements for fluoride determination in a variety of source water and drinking water.

Detection and clinical significance of apoptosis related protein in local advanced rectal cancer patients with preoperative neoadjuvant therapy / 中华外科杂志

<p><b>OBJECTIVE</b>To discuss the mechanism of rectal cancer apoptosis induced by preoperative chemoradiotherapy and evaluate its effect by detection of apoptosis related proteins in locally advanced colorectal cancer patients who had received preoperative chemoradiation.</p><p><b>METHODS</b>To detect Bcl-XL and Bax expression in rectal cancer before and after chemoradiotherapy by EnVision method, combined with patients clinical and pathological index, statistically analysis and evaluation their relationship and clinical significance.</p><p><b>RESULTS</b>Patients with or without tumor shrinkage after preoperative chemoradiotherapy was 13 cases and 21 cases. While the positive rate of Bcl-XL in rectal cancer before and after chemoradiotherapy were 58.8% (20/34) and 52.9% (18/34), respectively. There were significant difference between Bcl-XL change before and after chemoradiation with tumor size, tumor cells shrinkage and operation pattern. The positive rate of Bax in rectal cancer before and after chemoradiotherapy were 32.4% (11/34) and 44.1% (15/34), respectively. There were no significant difference between Bax change before and after chemoradiotherapy with tumor cells shrinkage. There were statistically significant difference between Bax ratio (χ(2) = 9.607, P = 0.048) before and after chemoradiation while there were no significant difference between Bcl-XL/Bax ratio before and after chemoradiation with tumor shrinkage. According to layered analysis with preoperative therapy, there were statistically significant difference (χ(2) = 13.964, P = 0.007) between Bcl-XL change with operation pattern while the same of significant difference between Bax change with tumor infiltration and tumor shrinkage (χ(2) = 10.806 and 10.455, both P < 0.05).</p><p><b>CONCLUSIONS</b>Preoperative chemoradiation can influence rectal cancer cell's apoptosis and treatment effect by changing Bcl-XL and Bax expression. Bcl-XL downregulation and Bax upregulation have shown important function in colorectal cancer cell apoptosis which induced by preoperative chemoradiation, it can also improve the effection of chemoradiation in rectal cancer.</p>

Quantitative assessment of blood perfusion in thyroid nodules by contrast-enhanced ultrasonography / 中华超声影像学杂志

Objective To assess the value of blood perfusion of thyroid nodules using contrastenhanced ultrasonography(CEUS). Methods Blood perfusion parameters of 78 preoperative patients with thyroid nodule were examined using CEUS. Microvessel density(MVD) in the thyroid nodule specimens was calculated by immunohistochemical staining using anti-factor CD34. Results Compared to papillary thyroid carcinoma (PTC) and nodular goiter (NG), there was significant differences in peak value(PEAK),maximum value of video signal intensity(Slmax), and mean value of video signal intensity(SImean) in thyroid nodule with diameter ＜ 1 cm ( P ＜ 0. 05 ), the MVD of thyroid nodules was not statistically significant ( P ＞ 0.05). There were significant differences in regional blood volume and MVD in thyroid nodule with diameter ≥ 1 cm between two groups ( P ＜ 0.05). Conclusions The characteristics of blood perfusion are dissimilar for thyroid nodules with different sizes and properties. The quantitative analysis with CEUS can provide valuable information for clinical diagnosis.

Relationship between the protein expression of P53, c-erbB-2, vascular endothelial growth factor and CD44 and the survival rates of stage II( colorectal cancer patients without radiochemotherapy after radical resection / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the relation between protein expression of 4 genes [P53,c-erbB-2,vascular endothelial factors(VEGF) and CD44]and survival rate in stage II( colorectal cancer(CRC) patients without radiochemotherapy after radical resection.</p><p><b>METHODS</b>One hundred and fifty-nine cases of stage II(CRC without radiochemotherapy were enrolled in this study. The clinicopathological date and 5-year follow-up data were reviewed. Streptavidin-peroxidase immunohistochemical technique was used to detect the expression of P53, c-erbB-2, VEGF and CD44 in formalin-fixed, paraffin embedded sections of CRC tissues from above 159 patients.</p><p><b>RESULTS</b>The 5-year survival rate was 82.4%. The rates of positive expression of P53, c-erbB-2, VEGF and CD44 were 58.5%(93/159), 26.4%(42/159), 57.9%(92/159) and 40.0%(54/159) respectively. The 5-year survival rates of positive expression patients were not significantly different with those of negative expression. chi(2) analysis showed that the positive expressions of 4 genes had no relationships with the prognosis.</p><p><b>CONCLUSION</b>The expression of 4 gene proteins has no relationship with the prognosis of stage II( CRC patients without radiochemotherapy after radical resection.</p>

Analysis of 90 autopsy cases in medical legal dispute / 法医学杂志

OBJECTIVE@#To analyze the significance of forensic autopsy in medical tangle.@*METHODS@#Ninety autopsy cases of medical legal dispute were retrospectively analyzed from the database of our department from 2001 to 2008. All cases were analyzed and classified based on age, sex, cause of death, clinic diagnosis and forensic diagnosis.@*RESULTS@#The age ranged from 1 day to 72 years, and the ratios of male to female is 1:1. The most common healthcare facilities involved were county hospitals (30 cases, 33.33%). The coincidence rate between clinical diagnoses and pathological diagnoses was 33.33%.@*CONCLUSION@#The forensic autopsy is valuable to solve or even avoid the occurrence of medical legal dispute.

Effects of curcumin on malondialdehyde and c-fos protein in hypoxia ischemia brain tissue in rats / 法医学杂志

OBJECTIVE@#To explore the effects of curcumin on the content of malondialdehyde (MDA) and the expression level of c-fos protein following hypoxia ischemia brain damage (HIBD) in rats.@*METHODS@#Sprague-Dauley (SD) rats were randomly divided into four groups as the following: sham group, hypoxia ischemia brain damage group, curcumin group and solvent control group. The content of MDA in the brain was measured by colorimetry. The expression level of c-fos protein in the cortex tissue was detected by immunohistochemistry. Morphologic and structural changes of neuron cells of the cortex were observed by electron microscopy.@*RESULTS@#The content of MDA was clearly lower in curcumin group than that in the other groups at the same time after HIBD. The expression level of c-fos protein was higher in the curcumin group than that in the other groups (P<0.05). Electron microscopy showed that the morphologic and structural changes of neuron cells of cortex in the curcumin group were reduced.@*CONCLUSION@#Curcumin could significantly decrease the content of MDA, increase the expression level of c-fos protein and reduce the damage of the neuron cells.

Acute necrotizing pancreatitis and postmortem autolysis of pancreas / 法医学杂志

OBJECTIVE@#To compare the pathomorphologic changes between the pancreas in acute necrotizing pancreatitis (ANP) and that in acute deaths of rats (within 48 hours) so as to find the distinctions.@*METHODS@#The animal models of ANP and other acute deaths (electroshock, mechanic asphyxia/strangle, and acute poisoning with tetramine) were established according to the criteria. Half-quantitative grading and image quantitative analysis methods were employed to observe the gross and microscopic changes of the pancreases.@*RESULTS@#Three features including inflammation infiltrate, fat necrosis and calcium deposit in the ANP group were considerably different from that in other acutely died rat group (P<0.05).@*CONCLUSION@#Inflammation infiltrate, fat necrosis and calcium deposit are the most important pathologic features found in ANP by common light microscope, distinguishing ANP from postmortem pancreatic autolysis.

Application of diatom detection using knead pulp method / 法医学杂志

OBJECTIVE@#A novel technology for detection of diatom was discussed.@*METHODS@#Five grams of testing sample were taken and the organics were removed using simple mechanical knead pulp method. The homogenized samples were concentrated by centrifugation, smeared, and then examined under light microscope.@*RESULTS@#Except for a few feather's grains, the vast majority of diatom could be identified easily with clear diatom striations. The organic diatom could also be easily detected by this methodology.@*CONCLUSION@#The detection of diatom using knead pulp method is not only simple and inexpensive with a higher successful rate, but also causes nearly no harm to human and environment.

Clinicopathological and related gene analysis in gastric adenocarcinoma and their correlation with prognosis / 肿瘤研究与临床

Scm in volume(42/60),multiple site involvement(44/60),blood type"O"(31/41),in comparison with those of survival group,and the difference was statistically significant.C-erbB-2,p16,p53,P-gp,CD_(44) and CD_(25)expression were not significantly different in these two groups. Conclusion The clinical stage, lymph node metastasis,lymphatic tumor emboli and/or neural involvement,infiltration depth,histological dif- ferentiation,tumor volume,involvement extension are important prognostic factors in patients with gastric can- cer,while the significance of cancer-related gene expression in gastric carcinomas needs to be studied further.

Effects of different dialysates on apoptosis and expression of PKC? of U937 cell line / 上海第二医科大学学报

Objective To investigate the effects of different dialysates on expression of protein kinase C-? (PKC?) and apoptosis of U937 cell line. Methods Different dialysates were added into culture fluid with U937 cell line at exponential phase of growth, and groups were divided: fluid A+fluid B group (dialysate A+dialysate B), fluid A+fluid B+rottlerin (PKC? specific inhibitor)group, fluid A+powder B group (dialysate A+powder B) and fluid A+powder B + rottlerin group. Besides, blank control group and normal control group were established. Cells were harvested 24 h and 48 h after treatment, morphological changes were observed by Hoechst33258 fluorescence staining, cell apoptosis was measured by Annexin-V-FITC/PI double staining, and expression of PKC? mRNA and protein was detected by RT-PCR and Western blotting, respectively. Results Cell apoptosis significantly increased in fluid A+powder B group, with typical morphology of apoptosis. After treatment for 24 h and 48 h, cell apoptosis rates in fluid A+powder B group were significantly higher than those at corresponding time points in blank control group, normal control group and fluid A+powder B+rottlerin group (P0.05). Conclusion Fluid A+powder B can significantly increase apoptosis of U937 cell line, the mechanism of which may be associated with the up-regulation of expression of PKC?. Compared with fluid A+powder B, fluid A+fluid B is superior in reducing apoptosis of peripheral blood monouclear cells.

Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion / 中国中药杂志

<p><b>OBJECTIVE</b>To study effect of triptolide (TL) on neuronal apoptosis in cerebral tissue of rat after ischemia-reperfusion.</p><p><b>METHOD</b>Triptolide at dose 0.2 or 0.4 mg x kg(-1) was intraperitoneally injected once a day for 4 d. The focal cerebral ischemia-reperfusion model was established with thread embolism in middle artery before triptolide injection on the fourth day. Neurological deficit score of rats was evaluated; and immunohistochemical techniques were used to count positive cells of express of MPO and TUNEL in cerebraltissue.</p><p><b>RESULT</b>Compared with the control group, the deficit of neural function was significantly improved, and the number of infiltrate of neutrophil and neuronal apoptosis in cerebral tissue was remarkably reduced in two TL-treated groups.</p><p><b>CONCLUSION</b>The results suggested that TL can inhibit infiltration of neutrophil and decrease the degree of neuronal apoptosis in cerebral tissue.</p>

A primary study on the ARP-SRP gene expression profiling of brain injury by cDNA microarray / 法医学杂志

OBJECTIVE@#To screen the differential expression of apoptosis-related protein and stress response protein(APR-SRP) genes after human brain injury by cDNA microrarray.@*METHODS@#The total RNAs were isolated from normal and injured brain tissues of a car-accident victim, and were purified to obtain mRNAs by Oligotex. Both mRNAs from the tissues of the injured and the normal tissue were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probes from normal tissue was labeled with Cy3-dUTP, that from the injured tissue with Cy5-dUTP. The mixed probes were hybridized to the BioDoor Chip ARP-SRP-1.0S, a cDNA microarray which contains 77 apoptosis related protein genes and 23 stress protein related genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues.@*RESULTS@#Among the 100 target genes, Only CLN2 gene (Homo sapiens lysosomal pepstatin insensitive protease gene) showed distinct deference in expression level between the brain injury and normal tissues.@*CONCLUSION@#cDNA microarray analysis indicated that CLN2 gene, which is correlated to a fatal childhood neurodegenerative disease, might be related to the brain injury. The expression level of CLN2 gene was significantly decreased in brain injured tissue in comparison to normal tissue. Further analysis of this gene will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine.

Study on the expression level of esophageal carcinoma P-gp and P27 and affecting survival in the patient / 中国癌症杂志

three years survival group,and those who died within 3 yrs had P-gp positive expression(24 cases,53.3%).Comparing of the two groups showed statistical significance(P3 year survival stage group,and the patients who died within three years group,the P27 expression was positive in 26 cases(56.5%)(P

Expressions of p53 and nm23 proteins in oral squamous cell carcinoma / 中国耳鼻咽喉头颈外科

Objective:To investigate the expressions of P53and nm23 proteins in cancer tissue of patients with oral squamous cell carcinoma.Methods:There were 57 males and 43 females,ranging in age from 35 to 75 years old.The clinical stages were T1 10,T2 29,T3 56 and T4 5;pathologic classifications were high differentiation in 74,medial and low differentiation in 26;lymph node metastasis in 56,no metastasis in 44.The expressions of P53 and nm23 proteins were detected by immunohistochemical technique.Results:The expressions rates of P53and nm23 proteins were 58.0%(58/100) and 40.0%(40/100) respectively (P＜0.05)in cancer tissue of patients with oral squamous cell carcinoma.The expressions rates of P53and nm23 were 71.5%(40/56)and 28.6%(16/56)in group of patients with node lymph node metastasis;40.9%(18/44)and 54.5%(24/44) in group of patients with negative metastasis respectively.Conclusion:Lymph node metastasis might be correlated with high level expression of P53 protein and low level expression of nm23 protein in patients of oral squamous cell carcinoma.

Sclerosing hemangioma: a clinicopathological study of lung / 肿瘤研究与临床

Objective To study the clinicopathologic characteristics of pulmonary sclerosing hemangioma(SH).Method Eleven cases of SH were studied by the clinical data and immunohistochemistry and review of the literature.Results SH has a characteristic architecture composed of round cell and surface cubic cell.It has a variety of tissular structure,mainly expression with four regions that contain solid regions,papillary regions,angiomatoid regions and sclerosing regions.Immunohistochemically,these two kinds of cells were both positive for TTF-1 and EMA.Conclusions SH is a tumor that originates from the epithelial cell of primary respiratory tract.Both the solid round cells and surface cubic cells are neoplastic.SH should differentiate with inflammatory,typeⅡpneumocyte papillary tumor,alveolus adenoma and typical carcinoid tumors.The parts of SH have invade grow tendency.Surgical cure of SH is suited for operating a lobe of the lung but not just peel of the tumor.