RESUMO
These active components and monomes inhibit thrombosis aimed directly at activation, adhesiveness and aggregation of platelet, thus preventing and curing ischemic cardiovascular and cerebrovascular diseases. Here we summarized the effect of active components and monomes of the traditional Chinese medicine targeted platelet on ischemic cardiovascular and cerebrovascular diseases, to provide references for drug investigation and clinical application.
Assuntos
Animais , Humanos , Alcaloides , Farmacologia , Medicamentos de Ervas Chinesas , Farmacologia , Flavonas , Farmacologia , Plantas Medicinais , Química , Ativação Plaquetária , Adesividade Plaquetária , Agregação Plaquetária , Terpenos , FarmacologiaRESUMO
<p><b>OBJECTIVE</b>To research the protective effect of pueraria flavonoid on the cerebral ischemic reperfusion injury.</p><p><b>METHOD</b>Using the middle cerebral artery occlusion model (MCAO) in rats, we investigated the influence of pueraria flavonoid on the brain water content, the infarct volume, the activities of SOD, and the content of MDA.</p><p><b>RESULT</b>Pueraria Flavonoid could obviously reduce the brain water content and the infract volume in MCAO, increase the activities of SOD, and decrease the content of MDA in the cerebral ischemia- reinfusion model of rats.</p><p><b>CONCLUSION</b>Pueraria has the function of scavenging free radicals and the protective effect on ischemic brain tissue.</p>
Assuntos
Animais , Feminino , Masculino , Ratos , Encéfalo , Metabolismo , Patologia , Flavonoides , Farmacologia , Infarto da Artéria Cerebral Média , Malondialdeído , Metabolismo , Fármacos Neuroprotetores , Farmacologia , Plantas Medicinais , Química , Pueraria , Química , Distribuição Aleatória , Ratos Sprague-Dawley , Traumatismo por Reperfusão , Metabolismo , Patologia , Superóxido Dismutase , MetabolismoRESUMO
Objective To isolate and identify the adult neural stem cells from the parenchyma of spinal cord in adult mouse.Methods The parenchymal spinal cord from adult mouse was dissected and dissociated by mechanical trituration.The tissue suspension was cultured in serum-free DMEM/F12 medium supplemented with EGF and B27.The cell colonies generated from a single cell were screened by limited dilution and incubated with BrdU.The cell colonies were transferred into medium with serum to induce differentiation.The cells were identified with antibodies to Nestin,BrdU,MAP2 and GFAP by immunofluorescence staining.Results The cells were cultured for seven days to generate proliferative neurospheres.The majority of cells in these neurospheres expressed Nestin and were differentiated into MAP2-positive cells and GFAP-positive cells in medium containing with fetal bovine serum.Conclusion A significant number of neural stem cells are present in the parenchymal adult mouse spinal cord and can proliferate and also give rise to neurons and glia in vitro.