Overall Evaluation and Management of 8 Instruments in Emergency Laboratory Using 6σ Quality Standard / 现代检验医学杂志

Objective To evaluate and improve overall quality detection of 8 instruments in emergency laboratory using 6σ quality standard.Methods In 2017,collected both internal quality control (IQC) and external quality assessment (EQA) data of 46 assay items from 8 instruments in their laboratory,and then calculated their σ values and total allowed errors ac cording to CLIA'88 standards.By comparison of their quality goal index (QGI) between first and second half year of 2017,successfully elevated assayquality using strategy (man,machine,material,method,measurment and enviroment).Results For all 46 assay items (except NT-proBNP without second EQA data),made better improvement of detection of 34 (75.6 ％ of 6σ qualified),38(84.4％ of 5σ qualified),40(88.9％ of 4σ qualified),44 (97.8％ of 3σ qualified) and 1 (2.2％ of 2～3σ qualified) items,respectively whereas there were 27 (58.7％ of 6σ qualified),30 (65.2％ of 5σ qualified),35 (76.1％ of 4σ qualified),41 (89.1％ of 3σ qualified) and 5 (10.9％ of 2～3σ qualified) items before improvement.Conclusion Using 6σ quality standard,could make more progress in the laboratory for many respects:fast and accurate clinical reports,reducing reinspection rate,and increasing patient's satisfactory,etc.

Research on pericellular matrix properties for chondrcytes / 中国骨伤

Pericellular matrix (PCM) is a narrow tissue region surrounding chondrocytes, which "chondron" with its enclosed cells. A number of studies suggested that PCM is rich in proteoglycans, collagen and fibronectin, and plays an important role in regulating microenvironment of chondrocytes. Direct measures of PCM properties through micropipette aspiration technique showed that PCM was different from mechanical property of chondrocytes and nature extracellular matrix. However, the function of PCM is not clear, and need further study.

Percutaneous kyphoplasty for the treatment of senior patient with acute and chronic osteoporotic vertebral compression fractures / 中国骨伤

<p><b>OBJECTIVE</b>To discuss the clinical effects of percutaneous kyphoplasty (PKP) for acute and chronic osteoporotic vertebral compression fractures (OVCF).</p><p><b>METHODS</b>From June 2006 to October 2011,42 senior patients with OVCF were treated with PKP. There were 5 males and 37 females,ranging in age from 60 to 86 years old with an average of (73.3+/-7.5) years old. The patients were divided into two groups (acute group and chronic group) according to the course,MRI and whether or not undergoing systematically conservative. VAS and ADL scoring systems were used to evaluate the pain and daily activities. X-ray was used to evaluate the vertebral height restoration rate and the kyphosis correction rate.</p><p><b>RESULTS</b>All patients were followed up from 10 to 64 months with an average of 17 months. At 1 week and 6 months after operation in two groups, VAS and ADL scores decreased significantly (P<0.05) ;VAS and ADL scores at 6 months after operation were higher than at 1 week after operation (P>0.05). But the improvement rates of the pain and daily activities of acute group were better than that of chronic group (P<0.05). At 1 week after operation,the restoration rate of anterior and middle vertebral height,the kyphotic correction rate in acute group were better than that of chronic group (P<0.05). CONCLUCSION: Using PKP to treat both acute and chronic osteoporotic vertebral compression fractures can get good effects,but acute patients can obtain better effects than chronic patients,it has obviously advantages of relieving pain,correcting kyphotic angle,recovering vertebral height.</p>

Progress on treatment of osteoporotic vertebral compression fracture / 中国骨伤

Osteoporosis vertebral compression fractures seriously affect the life and health of the elderly,indicating the elderly life health begin to decline. Therefore, aggressive treatment is particularly important. Traditional surgical method,such as laminectomy and internal fixation has large trauma,more blood loss and high failure rate. However, conservative treatment cannot early reduce pain symptoms and improve functional status,it can lead delayed union and severe ossification and form chronic OVCF,make more difficult to treat. In treatment of vertebral body fracture,there was a new way with the development of minimally invasive spinal surgery technology. Percutaneous vertebroplasty and percutaneous kyphoplasty has become the commonly used method in treating OVCF. With rapid and significant analgesic effect,vertebral body height recovered ideally, the patients can early load activities and preoperative complications is rare. Effective rehabilitation exercise is also important in treating OVCF by surgery at the same time.

Bio-panning of antagonistic peptides against HAb18G/CD147 and their function of anti-hepatoma invasion / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To screen out the HAb18G/CD147 binding peptides and find out an antagonist against hepatoma invasion.</p><p><b>METHODS</b>HAb18G/CD147 was purified by affinity chromatographic method and the antigen binding peptides acquired by bio-panning a phage-displayed 12-peptide library. After obtaining the sequence of the selected phage-displayed peptides, all the 9 peptides were synthesized by solid-phase method and identified by mass spectrograph. The peptides' anti-metastatic function was tested by Boyden Chamber assay.</p><p><b>RESULTS</b>The purified HAb18G/CD147, identified by Western blot (molecular weight about 65 kd) could be used to bio-pan the phage-displayed peptide library. After 3 rounds of bio-panning, 9 positive phage clones were selected and sequenced. The synthesized peptides had uneven inhibitory activities and three of them were able to markedly inhibit the hepatoma cell invasion (P < 0.01). The most effective peptide decreased by 90.1% of hepatoma cells migrating through the Boyden Chamber membrane as compared with the control.</p><p><b>CONCLUSION</b>Bio-panning the phage-displayed peptide library can be used successfully to screen out the antigen binding peptides. Hepatoma metastatic potential can be inhibited by peptide antagonist which could be a good foundation of developing peptide therapeutic agent against hepatoma metastasis.</p>