RESUMO
Objective To explore a newly discovered transmembrane protease serine 4 (TMPRSS4) isoforms and its molecular charcteristics in transfected colon cancer cells. Methods The named T4-1A and T4-1B of the TMPRSS4-isoforms were authenticated by the RT-PCR and Western blot,and then transfected to human colon cancer cells (DLD-1). Those stable transfected cells of migration and invasion were illustrated using wound healing assays and matrigel invasion assays. Results Successfully constructed T4-1A and T4-1B recombinant vectors,and obtained T4-1A and T4-1B transfected cell lines,and their T4-1A and T4-1B were highly expressed in stable DLD-1. Compared to cells transfected with empty vector of pcDNA6,the transfected DLD-1 with T4-1A enhanced migration and invasion were statistical significance (P < 0. 05). However,compared to pcDNA6 no significant1 difference was found for T4-1B. Moreover,the biological characteristics of T4-1A and TMPRSS4 were very similar. Conclusion The T4-1A and T4-1B is newly TMPRSS4-isoforms,and protease domain included to a T4-1A has further facilitated the migration and invasion of colon cancer cells,and further studies provide a theoretic base in the molecular biomedical characteristics of TMPRSS4.
RESUMO
Objective To investigate the relationship between diameter of early gastric cancer as well as lymph node metastasis and expression of vascular endothelial growth factor(VEGF) and Matrix metalloproteinases-7(MMP-7)in gastric cancer. Method VEGF and MMP-7 expressions of gastric cancer tissue in 55 cases were examined by immumohistochemical assay. The diagnosis was confirmed by mucous stain and pathology. Results The level of VEGF and MMP-7 expression in group of early gastric cancer with diameter ≤ 10 mm was significantly lower than that of diameter ≥ 10 mm and ≤ 20 mm (P0.05). Conclusion It is significant that relation of the diameter of early gastric cancer with VEGF and MMP-7 expression, the cases of strongly positive over-expression of both VEGF and MMP-7 in tissue of patients with early gastric cancer are accompanied by more likelihood of lymph node metastasis.