Effects of intelligent aerobic bicycle training on ischemic stroke patients at different disease courses / 中国康复理论与实践

ObjectiveTo investigate the effects of an intelligent aerobic bicycle training on lower limb motor function, cardiopulmonary function and activities of daily living (ADL) in patients with ischemic stroke at different courses. MethodsFrom November, 2019 to November, 2021, 138 ischemic stroke patients were stratified-randomly divided into control group (n = 69) and experimental group (n = 69). All the patients received medication and routine rehabilitation training, and the experimental group additionally received intelligent aerobic bicycle training, for eight weeks. They were assessed with Fugl-Meyer Assessment-Lower Extremities (FMA-LE), peak oxygen uptake (VO2peak), Six-minute Walk Test (6MWT) and modified Barthel Index (MBI) before, and four weeks and eight weeks after treatment. ResultsThe performances of FMA-LE, VO2peak, 6MWT and MBI improved in both groups after treatment (|t| > 6.763, P < 0.001), and improved more in the experimental group than in the control group (|t| > 2.439, P < 0.05). For the patients beginning training less than one month after stroke, the performances of FMA-LE, VO2peak and 6MWT improved more in the experimental group than in the control group (|t| > 2.036, P < 0.05); for those during one to three months, the performances of 6MWT and MBI improved more (|t| > 2.005, P < 0.05); for those during three to six months, the performances of all the tests were not significantly different (|t| < 1.303, P > 0.05). ConclusionIntelligent aerobic bicycle training may improve the lower limb motor function, cardiopulmonary function and ADL for patients with ischemic stroke, especially intervening earlier.

Inhibitory effect of peripheral blood iTr35 cells on vascular endothelial cell vascular cell adhesion molecule_1 in children with pulmonary artery hypertension / 中华实用儿科临床杂志

Objective To explore the role of iTr35 cells in the pathogenesis of children with pulmonary artery hypertension(PAH)in children,and the percentage of iTr35 cells and serum interleukin(IL)_35 levels in peripheral blood of patients with PAH were investigated. Their inhibitory effects on the expression of vascular cell adhesion mole_cule_1(VCAm_1)on vascular endothelial cells were also analyzed. Methods After 3 mL peripheral blood of 30 congenital heart disease( CHD)patients with PAH,22 CHD patients without PAH and 30 age or gender matched healthy controls(HC)were collected,the percentage of iTr35 cells were detected by flow cytometry and the concentra_tions of serum IL_35 were detected by Luminex,as well as soluble VCAm_1(sVCAm_1). Human pulmonary artery endothelial cells(HPAECs)were cultured in υitro and divided into control group,tumor necrosis factor( TNF)_α group and IL_35+TNF_α group. The expression of VCAm_1 and nuclear factor( NF)_κB P65 protein of each group were detected by flow cytometry and Western blot. The adhesion of peripheral blood mononuclear cells(PBmCs) to HPAECs was observed by fluorescence microscope. Results Compared to CHD patients without PAH,the percent_age of iTr35 cells[0. 86(0. 45_1. 63)% υs. 1. 14(0. 46_2. 11)%](H=20. 52,P<0. 05)in peripheral blood and serum IL_35 levels[2. 43(1. 76_2. 85)μg/L υs. 3. 17(2. 92_5. 66)μg/L]( H=119. 56,P<0. 05)were signifi_cantly decreased in CHD patients with PAH. However,serum sVCAm_1 concentration[923. 1(892. 6_1 118. 7)μg/L υs. 776. 1(743. 5_932. 3)μg/L ]in CHD patients with PAH were significant increased( H=65. 65,P<0. 05). In addition,the concentration of serum IL_35 were negatively correlated with sVCAm_1 in PAH patients(r= _0. 374 P=0. 042). In υitro,the positive rate of VCAm_1 on HPAECs was significant decreased in IL_35+TNF_α group as compared to the TNF_α group[(2. 07 ± 0. 82)% υs.(5. 83 ± 1. 34)%,F=1 197. 18,P<0. 05]. In addition,the protein expression of NF_κB P65 in HPAECs was significantly decreased in TNF_α+IL_35 group as compared to TNF_α group,as well as the adhesion of PBmCs to HPAECs(F=212. 04,2 533. 51,all P<0. 05). Conclusions The percentages of iTr35 and levels of IL_35 are reduced in the peripheral blood of patients with PAH. Through in υitro ex_periments,IL_35 is found to reduce PBmCs adhesion by inhibiting VCAm_1 expression in HPAECs.

Inhibitory effect of peripheral blood iTr35 cells on vascular endothelial cell vascular cell adhesion molecule-1 in children with pulmonary artery hypertension / 中华实用儿科临床杂志

Objective@#To explore the role of iTr35 cells in the pathogenesis of children with pulmonary artery hypertension (PAH) in children, and the percentage of iTr35 cells and serum interleukin(IL)-35 levels in peripheral blood of patients with PAH were investigated.Their inhibitory effects on the expression of vascular cell adhesion molecule-1 (VCAM-1) on vascular endothelial cells were also analyzed.@*Methods@#After 3 mL peripheral blood of 30 congenital heart disease (CHD) patients with PAH, 22 CHD patients without PAH and 30 age or gender matched healthy controls (HC) were collected, the percentage of iTr35 cells were detected by flow cytometry and the concentrations of serum IL-35 were detected by Luminex, as well as soluble VCAM-1 (sVCAM-1). Human pulmonary artery endothelial cells (HPAECs) were cultured in vitro and divided into control group, tumor necrosis factor (TNF)-α group and IL-35+ TNF-α group.The expression of VCAM-1 and nuclear factor(NF)-κB P65 protein of each group were detected by flow cytometry and Western blot.The adhesion of peripheral blood mononuclear cells (PBMCs) to HPAECs was observed by fluorescence microscope.@*Results@#Compared to CHD patients without PAH, the percentage of iTr35 cells[0.86(0.45-1.63)% vs.1.14(0.46-2.11)%](H=20.52, P<0.05) in peripheral blood and serum IL-35 levels[2.43(1.76-2.85) μg/L vs.3.17(2.92-5.66) μg/L](H=119.56, P<0.05) were significantly decreased in CHD patients with PAH.However, serum sVCAM-1 concentration[923.1(892.6-1 118.7) μg/L vs.776.1(743.5-932.3) μg/L ] in CHD patients with PAH were significant increased (H=65.65, P<0.05). In addition, the concentration of serum IL-35 were negatively correlated with sVCAM-1 in PAH patients (r=-0.374 P=0.042). In vitro, the positive rate of VCAM-1 on HPAECs was significant decreased in IL-35+ TNF-α group as compared to the TNF-α group [(2.07±0.82)% vs.(5.83±1.34)%, F=1 197.18, P<0.05]. In addition, the protein expression of NF-κB P65 in HPAECs was significantly decreased in TNF-α+ IL-35 group as compared to TNF-α group, as well as the adhesion of PBMCs to HPAECs (F=212.04, 2 533.51, all P<0.05).@*Conclusions@#The percentages of iTr35 and levels of IL-35 are reduced in the peripheral blood of patients with PAH.Through in vitro experiments, IL-35 is found to reduce PBMCs adhesion by inhibiting VCAM-1 expression in HPAECs.

Antiviral activity determination of recombinant equine interferon-gamma and identification inhibited antiviral activity of monoclonal antibodies / 生物工程学报

Equine interferon-gamma (eIFN-gamma) expressed both in E. coli and baculovirus were evaluated for antiviral activity against recombinant Vesicular Stomatits Virus expressing green fluorescence protein (rVSV-GFP) in EFK-78 cells. The assays were conducted in 96-well plate. Virus infectivity was measured by quantifying GFP-positive cells, instead of quantifying the CPE reduction. Prior to infection of EFK-78 cells with rVSV-GFP, the cells were incubated with eIFN-gamma. The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with eIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titers of antiviral activity were 1 x 10(3) AU/mL and 1 x 10(5) AU/mL of eIFN-gamma expressed from E. coli and baculovirus, respectively. The antiviral activities of the recombinant eIFN-gamma were highly efficient and specific, as it was blocked by mAbs against eIFN-gamma.

The applied research on neural network filtrated by rough-set in insect taxonomy / 生物医学工程学杂志

This article provides demonstrations and calculations, using rough-set theory and method, of the math-morphological features (MMFs), such as form parameter, lobation and sphericity, etc. drawn from 28 species of insects of the Hemiptera, Lepidoptera and Coleoptera based on their images. The results are compared with statistical analysis made by Zhao Hanqing, and also with the traditional classifications through the pattern recognition of neural network on the basis of the rough-set disposal. The result of the experiments showed that when used in categorical taxonomy, the importance of MMF was ranked from high to low: (roundness-likelihood. eccentricity) > (hot-hole number, sphericity, circularity) > (lobation, form parameter). The results of pattern recognition by neural network were completely identical with those of traditional classifications. Accordingly, the conclusion was that this theory applied in insect taxonomy was more idealistic compared with statistical analysis method, and it had great significance when used with rough-set neural network.