Comparison between the quantitative and semi-quantitative fluorescence RT-PCR assays for the detection of XDH/XO mRNA expression in different organ tissues of rhesus monkey / 中国比较医学杂志

Objective To analyze the differences between the semi-quantitative RT-PCR and real time quantitative fluorescence RT-PCR assays for detecting XDH/XO mRNA expression in various organ tissues of rhesus monkey, and provide useful reference in methodology of experimental studies.Total RNA was extracted from the myocardium, kidney, testis, skin, and liver tissues, respectively, for detecting XDH/XO mRNA expression in rhesus monkey by semi-quantitative RT-PCR and real time quantitative fluorescence RT-PCR assays.The sensitivity and specificity of the two assays were compared with each other using the same primer sequences and reference genes.Results The expression of XDH/XO mRNA in different organ tissues were detected by both the two PCR assays.The sensitivity of quantitative fluorescence real-time RT-PCR for the XDH/XO mRNA expression in the liver tissue was 39 times higher than that by semi-quantitative RT-PCR.Conclusions Both the quantitative and semi-quantitative fluorescence RT-PCR assays can be used to detect the expression of XDH/XO mRNA in different organ tissues of rhesus monkey.The sensitivity of quantitative fluorescence real-time PCR assay is more sensitive than that of the semi-quantitative RT-PCR assay.

Metabolic nuclear receptors and metabolic syndrome / 基础医学与临床

Metabolic nuclear receptors consist of a group of nuclear hormone receptor transcription factors including peroxisome proliferator-activated receptors(PPARs),liver X-activated receptors(LXRs) and farnesoid X-activated receptors(FXRs).They have been identified and drawn enormous attention due to key roles in regulating adipogenesis,lipid metabolism,energy metabolism,insulin sensitivity,inflammation,blood pressure,cell growth and differentiation.Growing evidences support a causative relationship between these three metabolic nuclear receptors and the metabolic syndrome,which is characterized by insulin resistance,glucose intolerance or type II diabetes,obesity,dyslipidemia,hypertension,and microalbuminuria.These receptors have also been implicated in pathogenesis of atherosclerosis.Here we review the literature pertaining to the action and physiological role of metabolic nuclear receptors with particular emphasis on their pathogenic roles in the metabolic syndrome.