Relationship between amount of HBV DNA in serum/liver tissue and hepatitis G virus (HGV) infection in patients with chronic hepatitis B / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To observe the relationship between the amount of HBV DNA in serum/liver tissue and HGV infection in patients with chronic hepatitis B (CH-B) for exploring the effect of HGV infection on hepatitis B virus (HBV) replication of CH-B.</p><p><b>METHODS</b>HGV RNA in serum, HGV nonstructural region 5 (NS5) antigen (HGV Ag) in liver tissue and the amount of HBV DNA in serum, liver tissue were detected for 56 patients with CH-B by reverse transcription-polymerase chain reaction (RT-PCR) assay, peroxidase antiperoxidase (PAP) immunohistochemical method and fluorescence quantitative PCR assay, respectively. Then the relationship between HGV Ag expression in liver tissue and HGV RNA expression in serum was analysed and the amount of HBV DNA in serum and liver tissues from the serum HGV RNA or liver tissue HGV Ag positive patients were compared with those of the serum HGV-RNA or liver tissue HGV Ag negative patients, respectively.</p><p><b>RESULTS</b>Ten (17.9%) and eight (14.3%) patients were positive for serum and liver tissues,respectively.HGV RNA expression in serum was closely related to HGV Ag expression in liver tissues, but there was HGV RNA in serum from some of the liver tissues HGV Ag negative patients ?cases of HGV RNA and HGV Ag positive or negative,HGV RNA positive but HGV Ag negative, HGV RNA negative but HGV Ag positive, respectively: 5,43,5,3,(P<0.01). There was no significant difference in the amount of HBV DNA in serum and liver tissues between HGV RNA or HGV Ag positive and negative patients (P>0.05).</p><p><b>CONCLUSIONS</b>HGV infection may not affect HBV replication. Liver is the site of HGV replication, but HGV probably also replicates in extrahepatic tissues. HGV hepatic pathogenicity is probably mild and further studies are still needed.</p>

STUDY OF THE ETIOLOGICAL SIGNIFICANCE OF HBV IN PRIMARY HEPATOCELLULAR CARCINOMA / 解放军医学杂志

The clinical data and HE stain sections of 70 PHC cases and 7 bile-capillary carcinoma patients were reviewed. Victoria blue stain was used for all these liver samples,. Our observations showed that the detection rates of HBsAg in liver cells without a history of positive serum HBsAg test and either with serum HBsAg negative or positive were rather high, 85.7%. 75.0% and 90.2% respectively. There was no significant difference among them. It is worthy to note that HBsAg could be detected in liver cells sections with Victoria blue stain in 75.0% of liver samples whose serum HBsAg was negative. The positive rates of HBsAg in liver cells of serum HBsAg negative and positive patients were without obvious difference. It seemed to suggest that the posibility of HBV infection and its carcinogenic role could not be excluded in the serum HBsAg negative patient. In addition, HBsAg could be detected in the carcinoma cells in 34.3% of liver samples, and also in the abnormal cells between the carcinoma and liver tissue sections in 9.8% with Victoria blue stain. However, neither HBsAg could be found in all the 7 bile-capillary carcinoma sections with in Victoria blue stain, norground-glass cells could be seen in the HE stain sections. These results seemed to further prove that there might be a close cause and effect relationship between the HBV infection and PHC.

SURVEY ON THE TREATMENT OF 200 CASES OF CHRONIC HEPATITIS B WITH SPECIFIC TRANSFER FACTOR EXTRACTED FROM HBVM POSITIVE PLACENTA / 解放军医学杂志

The excellent results of 200 cases of chronic hepatitis B (CAH 167,CPH 16.CAH with liver cirrhosis tendency 11 and hepatitis-cirrhosis 6) treated with specific transfer factor extracted from HBVM positive placenta (PSTF) for 3 months in average.The clinical recovery rate was 83.0%,improvement 11.0%.unrecovery only 6.0%,and seroconversion of HBsAg and HBeAg was 9.9% and 32.9% respectively in the near-future.No sider-effect was found during treatment.The relapse rate was 13.9% during average 5.5 months follow-up after discharged,and the clinical recovery rate of the improved and unrecovered cases while they discharged from the hospital was 40.0% and 50.0% respectively during follow-up Therefore,the authors think that the PSTF perhaps an exellent agent similar to SSTF (specific transfer factor extracted from HBVM positive spleen) in treating chronic hepatitis B.But,owing to easiness to collect HBVM positive placenta in our country at present,PSTF perhaps could make a great offer for treating chronic hepatitis B,if it could be used correctly,and,therefore/it seems worthy for profound investigation.

A STUDY ON RELATIONSHIP BETWEEN HBV INFECTION AND LIVER CIRRHOSIS / 解放军医学杂志

It was observed that in 81.5% of 140 cases with liver cirrhosis, the serum was HBVM positive, and the liver biopsy showed characteristic features of CAH. HBsAg could be detected with VB stains in 77.6% of HBVM seropositive cases, 66.7% of HBsAg seronegative cases, and 60.0% of the cases whose serum HBVM had not been examined. There was no significant difference among them statistically. It was our belief that negative serum HBsAg did not rule out the posibility of HBV infection in liver cirrhosis patients. Our observations suggested that there might be a very close relationship between HBV infection and liver cirrhosis in our country, and liver cirrhosis was the outcome of HBV infection. Therefore, in the prevention of liver cirrhosis it should be emphasized that HBV infection should be prevented and CAH well treated.