Metabolic study of ginsenoside Re in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To systemically study the metabolism of ginsenoside Re in rats.</p><p><b>METHOD</b>Six SD rats were used divided into 3 groups. After oral administration of ginsenoside Re at a dosage of 100 mg x kg(-1), feces were collected at 12, 24, 36, 48, and 60 h. The microbial transformation metabolites of ginsenoside Re were used as standard references. HPLC-ESI-MS/MS analysis was used in the metabolism studies.</p><p><b>RESULT</b>Six metabolites of ginsenoside Re were detected in rat feces by HPLC-ESI-MS/MS analysis. Their structures were identified as 20(S)-ginsenoside Rg2, 20(S)-ginsenoside Rh1, 20(R)-ginsenoside Rh1, ginsenoside F1, 3-oxo-ginsenoside Rh1 and protopanaxatriol.</p><p><b>CONCLUSION</b>Ginsenoside Re has the same metabolic pathway with microorganisms, to some extent, justified the use of microbial models for mammalian metabolism studies.</p>

Distribution study of two constituents in rat after oral administration of Smilax china extract / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the distribution in rat after oral administration of Smilax china extract.</p><p><b>METHOD</b>Oxyresveratrol and resveratrol were quantitatively determined by HPLC/DAD method in rat tissues including heart, liver, spleen, lung and kidney after oral administration of S. china extract. The separation was performed on a Zorbax SB-C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water using linear gradient elution and UV detection at wavelength of 320 nm. The flow rate was 1.0 mL x min(-1) and the column was kept at 40 degrees C.</p><p><b>RESULT</b>The HPLC/DAD method for the simultaneous determination of oxyresveratrol and resveratrol in rat tissues was developed.</p><p><b>CONCLUSION</b>The method has been successfully applied for the distribution study of two active constituents in rat through oral administration of S. china extract.</p>