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To increase detection sensitivity and specificity on hepatitis C virus (HCV) is vital for prevention and controlling of the disease. To establish a more reliable detection method for HCV diagnosis, the full gene fragment of ns3 (non-structural protein of HCV) from recombinant plasmid of J6/JFH1 2a was amplified and then connected into the pET-28a prokaryotic expression vector, and the latter was subsequently transformed into Escherichia coli BL21 (DE3) to have the target protein expression. As a result, a protein with a molecular weight of 72 kDa was obtained and visualized in 10% SDS-PAGE. The purified NS3 protein was used as immunogen to inoculate BALB/c mice and the sera was collected after the fourth immunization. The antibody titer of serum is determined to be about 1:256000 with ELISA. Western blotting and indirect immunofluorescence analysis showed that the mouse polyclonal antibody could react specifically with the native NS3 protein in Huh 7.5.1 cells infected with HCV. These findings may provide basis for further preparation of monoclonal antibodies against NS3 and the development of related detection kit.
Assuntos
Animais , Camundongos , Anticorpos Antivirais , Alergia e Imunologia , Ensaio de Imunoadsorção Enzimática , Escherichia coli , Hepacivirus , Camundongos Endogâmicos BALB C , Plasmídeos , Proteínas não Estruturais Virais , Alergia e ImunologiaRESUMO
At present clinic study and use of anti-tumour platinum drug are wide and active. The paper reviews progression of clinic study and use of anti-tumour platinum drug and expects its prospect.
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Targeted drug is becoming a hot point for anti-tumor research.The paper reviews the cate- gory,speciality,principle of action and clinical application.The review also focus on the advance of anti-tu- mor targeted drug and forsee the prospect of it.
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Objective To study the inhibitory effect of yan xiao capsules on hepatocarcinoma mice. Methods Forty mice were randomly divided into control group ,CTX group,ADM group,trial group. Observe tumour weight ,the span time of tumour-bearing mice, tumour morphology and the mortality rate of ascites cancer cell by direct killing respectively. Results The tumour weight markedly decreased in trial group, the rate of inhibition tumour was 52.0 %(P