RESUMO
Objective To investigate the etiologic factors of chronic prostatitis following sexually transmitted urethritis. Methods The pathogens have been detected in 86 patients with chronic prostatitis following sexually transmitted urethritis. Results 57 strains (66.3%) of bacteria were cultured in expressed prostatic secretions(EPS) from 86 patients,in which gonococcus was the majority of cultures,accounting for 38.4%. Chlamydia trachomatis (CT), ureaplasma urialyticum (UU) and gonococcus were also detected by polymerase chain reaction (PCR) in expressed prostatic secretions,25.6%(22/86), 22.0%(19/86),and 43.0%(37/86) showed positive results respectively. Conclusions Gonococcus,chlamydia trachomatis and ureaplasma urialyticum were the main pathogens of chronic prostatitis.The results indicate that pathogen examination plays an important role in treatment of chronic prostatitis following sexually transmitted urethritis.
RESUMO
Objective To disclose the influence of PTEN on the migration of breast cancer cells ZR-75-1. Methods Wild-type PTEN gene was transtected into ZR-75-1 breast cancer cells which lack PTEN gene, tranfected cells were selected by puromycin and the protein encoded by PTEN gene was tested by Western-blot. The inhibition rate of invasion and adhesion of ZR-75-1 was tested on reconstituted basement membrane. Results Wild-type PTEN gene was successfully transfected into ZR-75-1 and expressed efficiently. The inhibition rate of invasion and adhesion is 70.4 % and 60.0 % respectively. Conclusion PTEN gene can restrict the migration of breast cancer cells in some degree, so whether PTEN gene is deleted or not can partly estimates the risk of migration of breast cancer cells.