Isolation-preparation and determination of chromones from Saposhnikovia divaricata / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.</p><p><b>METHOD</b>The separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>All the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.</p>

Content comparison of diterpenoid pigments in different processed products of Gardenia jasminoides / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the contents of diterpenoid pigments in the different processed products of Gardenia jasminoides.</p><p><b>METHOD</b>The separation of Crocin 1, Crocin 2, Crocin 3, Crocetin were determined simultaneously by HPLC on a kromasil C18 column at 35 degrees C with the m methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrase. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>The obtained linearity of the four components was better over 0.9995 and the average recoveries were 97.77%, 100.05% , 98.40%, 101.02%, respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate with good reproducibility. The results showed that the remarkable variation regulations appear among the different processed products.</p>

Comparative studies on contents of iridoid in processed products from Gardenia jasminoides / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the contents of iridoid in the different processed products from Gardenia jasminoides.</p><p><b>METHOD</b>Contents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12:88) as the mobile phrase. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>The obtained linearity of the two components was good with that the relative coefficients (r) were over 0.9994 and the average recoveries were 101.8%, 99.1%, respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate with good reproducibility. The results showed contents of two components are difference in the different processed products.</p>