RESUMO
<p><b>OBJECTIVE</b>To analyze the genetic polymorphism of 15 single nucleotide polymorphism (SNP) loci on the nonrecombining portion of the Y chromosome in 6 populations in China.</p><p><b>METHODS</b>Allelic specific polymerase chain reaction and 2% agarose gel electrophoresis and 6% PAGE were used to analyze the genetic polymorphism of 343 unrelated males, representing 6 populations in China, including Fujian Hans, Sichuan Hans, Mongolian, Hezhen, Sibo and Hui from the South, Northeast and Northwest.</p><p><b>RESULTS</b>Thirty haplogroups were observed, and 3 of them (H15, H16, H18) were seen in all of the six populations. Although the heterozygosity levels of the Hezhen, Mongolian, Sibo populations are similar and those of the other 3 populations (Fujian Hans, Sichuan Hans, Hui) are similar, the pairwise differences among haplogroups are significant. Analysis of molecular variance (AMOVA) and principal component (PC) analysis of the haplogroup distributions suggested highly different allele diversity between group I including Hezhen, Mongolian, Sibo and group II including Hui, Fujian Hans, Sichuan Hans.</p><p><b>CONCLUSION</b>The above analyses show more significant variance components in Northeast/South populations and clearly reveal the geographic genetic relationship among the six populations in the Northeast/Northwest/South. These results confirm the complexity of the genetic structure of Chinese populations and make a significant contribution for constructing the contemporary human gene pool and tracing genetic dispersal trail from Chinese populations.</p>
Assuntos
Humanos , Alelos , China , Etnologia , Cromossomos Humanos Y , Variação Genética , Genética Populacional , Polimorfismo de Nucleotídeo ÚnicoRESUMO
<p><b>OBJECTIVE</b>To evaluate the potential of p53 gene therapy for lung cancer in nude mice.</p><p><b>METHODS</b>Two lung adenocarcinoma cell lines L-18 and 95D were infected with adenovirus encoding wild-type p53 gene pAdCMV -p53 (Ad-p53 ) in vitro and in vivo. The antitumor effect of wild type p53 gene was assessed by cell growth curve, reverse transcriptase polymerase chain reaction (RT-PCR) analysis and TUNEL staining methods.</p><p><b>RESULTS</b>The p53-specific growth inhibition and apoptosis of tumor cells were observed in both cell lines in vitro. By RT-PCR analysis, the increasing expression of p21 gene but not of p16 gene after p53 gene infection suggested that p21 gene played an important role in p53 gene induced cell apoptosis. The in vivo study revealed that celiac injection of p53 gene significantly inhibited the tumorigenesis in 95D and L-18 cells in nude mice. However, no obvious inhibition of tumorigenesis was observed after subcutaneous injection of p53 gene in L-18 cell line, compared with the inhibition noted in 95D cell line.</p><p><b>CONCLUSION</b>The results showed the adenovirus-mediated antitumor therapy by means of p53 gene infection might be a potential way to inhibit cancer growth and induce tumor cell apoptosis.</p>
Assuntos
Animais , Camundongos , Adenocarcinoma , Genética , Patologia , Adenoviridae , Genética , Apoptose , Genética , Divisão Celular , Genética , Inibidor p16 de Quinase Dependente de Ciclina , Genética , Regulação Neoplásica da Expressão Gênica , Vetores Genéticos , Genética , Marcação In Situ das Extremidades Cortadas , Neoplasias Pulmonares , Genética , Patologia , Camundongos Nus , Transplante de Neoplasias , Proteína Oncogênica p21(ras) , Genética , Transfecção , Células Tumorais Cultivadas , Proteína Supressora de Tumor p53 , GenéticaRESUMO
<p><b>OBJECTIVE</b>To study the growth inhibitory effects of p21WAF1 and p53 overexpression in human lung adenocarcinoma cell line.</p><p><b>METHODS</b>The p21WAF1 and p53 gene were transfected respectively into a human lung adenocarcinoma cell line, GLC-82. Flow cytometry (FLC), transmission electron microscopy (EM) and TUNEL technique were used to evaluate cell growth and identify apoptosis.</p><p><b>RESULTS</b>The GLC-82 transfected by p21 plasmid showed increased cell number in G1 phase of cell cycle, decreased proliferation potential and decreased cloning efficiency. Apoptosis have not been detected neither on EM nor by TUNEL technique, whereas the GLC-82 infected by Ad-p53 showed significantly decreased proliferation potential and some of them even died, in addition apoptosis was confirmed by TUNEL technique.</p><p><b>CONCLUSION</b>The results indicate that p21WAF1 and p53 can inhibit proliferation; p53 also can induce apoptosis of lung adenocarcinoma cell. Therefore, these two genes should have a wide application in gene therapy of tumors in future.</p>