Isolation and cultivation of keratinocyte stem-like cells from human skin dermis / 中国组织工程研究

10.3969/j.issn.2095-4344.2013.23.012

Correlation between environmental factors and liposoluble and hydrophilic constituents of Polygalae Radix / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the correlation between environmental factors and the lipophilic and hydrophilic constituents of Polygalae Radix.</p><p><b>METHOD</b>The contents of lipophilic constituent were determined by GC-MS and hydrophilic constituents by HPLC. Geographical factors were collected by on-site inspection and climate factors by the local meteorological data. The relationship between the content of the lipophilic and hydrophilic constituents and the factors were analyzed by SPSS 18.0.</p><p><b>RESULT</b>There was linear relationship between the content of lipophilic constituent and climate factors such as average temperature of July, average temperature of January. There was also linear relationship between hydrophilic constituents and climate factors such as annual average temperature, latitude, annual average rainfall.</p><p><b>CONCLUSION</b>The main climate factors that affect liposoluble constituent content were average temperature of July, average temperature of January, and the main climate factors that affect hydrophilic constituent content were annual average temperature, latitude, annual average rainfall. The study would contribute to the quality evaluation and suitability of origin of Polygalae Radix.</p>

Germplasm resources of Polygala tenuifolia and determination of presenegenin in processing products / 中国中药杂志

<p><b>OBJECTIVE</b>To research the germplasm resources and the contents of senegenin in processing products of Polygala tenuifolia.</p><p><b>METHOD</b>The contents of senegenin in wild Polygala tenuifolia and cultivated samples of Polygala tenuifolia were determined by RP-HPLC, and compared.</p><p><b>RESULT</b>The contents of senegenin in wild reduce gradually along Shaanxi, Shanxi, Hebei to Dongbei. The contents of senegenin in cultivated three-year samples of three year Polygala tenuifolia from five main place was similar, 0.44%-0.49%. The content of senegenin were 0.44%-0.64% in the wand and 0.03%-0.09% in the residual part of stem, and the content of senegenin in Polygala tenuifolia was more than that in processing products.</p><p><b>CONCLUSION</b>There is a correlation between the content of senegenin in Polygala tenuifolia and ecology environment that show a is inverse proportion with the quality grade, and the contents in the processing products were decreased. Senegenin can be used as a characteristic marker in range. This research provides a reference for search a index for quality control of Radix polygala and its processing products.</p>

The Medicinal Value of Gold and Silver / 国际中医中药杂志

On the basis of extensive study of literature,we summed up the medicinal value and pharmacological functions of gold and silver,and put forward our views on the further research and development of these metals.

Data mining for simple sequence repeats in expressed sequence tags from Saruma henryi / 中草药

Objective To analyze the simple sequence repeat(SSR)information in expressed sequence tag(EST)resource of Saruma henryi and lay a solid foundation for the development of EST-SSR markers in this species.Methods ESTs of S.henryi were downloaded from GenBank and used to perform the contig assembly using Sequencher 4.8.Uni-ESTs were obtained and screened for SSR-containing unigenes using SciRoKo 3.4.The distributing frequency of the EST-SSRs and the basic characteristics of motifs were analyzed.Results A total of 10 274 ESTs of S.henryi were retrieved and were assembled into 6 643 non-redundant Uni-ESTs with a total length of 5.11?106 bp.In all,the data mining yielded 1 408 SSR loci,which corresponded to 1 232 Uni-ESTs(18.55%).On average,EST-SSRs spanned 22.30 bp,and occurred every 3.63 kb in length.In S.henryi,mononucleotide repeats predominated with an occurrence frequency of 12.24%.Dinucleotide repeats followed with a frequency of 5.01%.The most frequent one was A/T among all the repeat motifs,then followed by AG/CT.Conclusion SSRs in ESTs of S.henryi display a relatively high level of occurrence frequency and show abundance of types.