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Objective To investigate the relationship between C-peptide and artherosclerosis in lower ex-tremity in patients with type 2 diabetes. Methods Two hundred and fourteen type 2 diabetic patients were included in this retrospective study. They were divided into two groups:type 2 diabetes with artherosclerosis in lower extremi-ty in 123 cases and type 2 diabetes without artherosclerosis in lower extremity in 91 cases. Their medical history and the laboratory data were collected such as fasting serum C-peptide levels and postprandial serum C-peptide levels of 1 hour, 2 hours and 3 hours, glycated hemoglobin (HbA1c) and so on. Results Compared with artherosclerosis group in lower extremity, the age, duration of diabetes, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), glycated hemoglobin and smoking rate were significantly higher in type 2 diabetes without artherosclero-sis in lower extremity (P 0.05). Logistic regression analysis showed that fasting and postprandial (1 hour, 2 hours and 3 hours)serum C-pep-tide levels were all negatively related to artherosclerosis in lower extremity (OR 0.524,P = 0; OR 0.440, P = 0;OR 0.688, P=0;OR 0.795, P=0). Conclusions Serum C-peptide level may be an independent associated fac-tor with artherosclerosis in lower extremity in type 2 diabetes.
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Objective To investigate the expression of matrix metalloproteinase-2(MMP-2) in the VX2 hepatic tumor.Methods 30 New Zealand white rabbit VX2 liver carcinoma models(all the tumors were verified by histopathology)were set up.The expressions of MMP-2 in 30 cases of VX2 tumor tissues and their pericarcinomatous tissues and normal hepatic tissues in 20 cases were detected by immunohistochemical staining with SP method at 2.5 weeks after implantation.The cell number of MMP-2 positive expression was counted by immunohistochemical stain.Results According to immunohistochemical results,the positiveness of MMP-2 was 86.7%(26/30) in tumor tissues and 53.3%(16/30) in pericarcinomatous tissues and no expressions of MMP-2 in normal hepatic tissues.The expression of MMP-2 were mainly in the tumor cytoplasm and intercellular substance,and also in the part of the tumor thrombi,the bile vessel epithelial cell and vascular endothelial cell.Conclusion The features of the expression of MMP-2 are similar to that of human HCC,and this model is a real animal model for clinical MMP-2 study of human HCC.
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Objective To explore the effect of transcatheter arterial chemoembolization ( TACE ) on the apoptosis proteins fas,bax,and bcl-xl in hepatocellular carcinoma ( HCC ) . Methods Sixty-three cases with HCC proved by histopathologically were studied, which included 42 cases treated with surgical resection alone,and 21 cases underwent TACE . The expressions of fas,bax,bcl-2 and bcl-xl were detected with immunohistochemical SP method .Results The positive rate of fas was 47.62% ( 10/21 ) in HCCs treated with TACE,and 21.43%(9/42) in those treated with surgical resection alone, there was significantly difference between the two groups(?0.05). Conclusion TACE can increase the expression of fas and decrease the expression of bcl-xl, it can increase the rate of apoptosis of HCC, make HCC lesion contract. The effect of TACE to bax need more research.