The Interaction of 5-Fluorouracil with Deoxyribonucleic Acid and Serum Albumin / 分析化学

The interaction of 5-fluorouracil (5-FU) with deoxyribonucleic acid (DNA) and serum albumin and the influence of some metal ions on the interaction have been studied by using spectral methods of UV-absorption,fluorescence and circular dichroism. It was shown that the interaction of 5-FU with DNA and serum albumin was effected by some metal ions. The binding costant of 5-FU with serum albumin was found to be KHSA = 4.38 × 103(mol/L)-1 and KBSA =4.95 × 103(mol/L)-1 and the distance between the bound 5-FU and the tryptophan residue was also determined to be rHSA = 3.53 nm, rBSA = 3.51 nm.