RESUMO
In this study, the development of mycelium of Helvella crispa (Scop.) Fr. which added the different concentration of glucose and lactose to potato dextrose agar and malt extract agar. For this; glucose and lactose have been added as 0.5, 1.5 and 2.5% concentration to potato dextrose agar and malt extract agar separately. The radial growth speed, morphologic characterizations and pigmentation of mycelium were taken as criteria. The colonization period of mycelium in potato dextrose agar and malt extract agar was completed in 11 and 15 days approximately. Mycelium developed as dense at the potato dextrose agar medium with glucose (at the 0.5 and 1.5% concentration) and developed very dense with glucose (at the 2.5% concentration). But mycelium developed as dense at the malt extract agar medium with lactose (at the 0.5 and 1.5% concentration) and developed very dense with lactose (at the 2.5% concentration). The development of the mycelium was obtained as linear at the both two agar medium. Mycelium has formed yellowish-brown pigmentation.
RESUMO
In this study, antifungal activities of Pseudoclitocybe cyathiformis extracts with the help of acetone and chloroform against to Fusarium species (Fusarium culmorum and Fusarium moniliforme) were investigated. Pseudoclitocybe cyathiformis was dried at aseptic conditions and put thru extractions for 12 hours in solvents. Than the evaporator at 40°C and finally dried material stored at + 4°C.(Jonathan and Fasidi, 2003). Antifungal activities were measured by Disc Diffusion method (Stoke and Ridgway, 1980). According to this method; the inoculums containing Fusarium culmorum and Fusarium moniliforme were spread agar medium. In result antagonistic effects of Pseudoclitocybe cyathiformis was found against both Fusarium spp.. The clear zone of inhibition was observed around the mushroom extracts. For control water and only acetone and chloroform saturated discs were used. The results were comprised with commercial antibiotics (amoxycillin and erythromycin).
RESUMO
The aim of this study was to investigate the potential radioprotective effect of kombucha musroom tea (KM – tea) on gamma radiation (g) – induced chromosomal aberrations (CAs) in human peripheral blood lymphocytes in vitro. For this purpose, we used in vitro dose-effect relationship, and correlated these data with statistical parameters. CAs were classified into six major types as break, dicentric, acentric, fragment, gap and ring. Mitotic index (MI) and the numbers of aberrant metaphases (AMN) were also calculated for each donor. Six groups of the lymphocytes were prepared by in vitro culture according to the standard protocol. Group I (control) did not receive any g – radiation or KM – tea, Group II (positive control) was treated with 1000 μl dose of KM – tea alone, Group III was treated with 5 Gy dose of g – radiation alone, Group IV was treated with 250 μl dose of KM – tea before irradiation, Group V was treated with 500 μl KM – tea before irradiation, Group VI was treated with 1000 μl KM–tea before irradiation. The results indicated that all KM–tea supplemented lymphocytes had lower frequency of CAs than in the group treated with g - radiation alone (p<0.05). It was seen that KM – tea had a protective effect againist CAs particularly at 500 and 1000 μl doses. Besides, MI values increased and AMN decreased after application of KM – tea in a dose/ dependent manner. In vitro results showed that KM – tea supplementation may decrease the frequency of CAs and its radioprotective action against ionizing radiation is dose-dependent.