RESUMO
<p><b>OBJECTIVE</b>To assess the association of TLR4 gene polymorphisms with large artery atherosclerosis (LAA) stroke and liability to atherosclerosis in an ethnic Han population from northern China.</p><p><b>METHODS</b>The study has involved 286 LAA stroke patients and 300 healthy controls. The LAA group has been divided 4 subsets according to angiostenosis conditions. Polymerase chain reaction-restriction fragment length polymorphism and pyrosequencing were employed to analyze three single nucleotide polymorphism (SNPs) (rs1927914, rs1927911 and rs2149356) of the TLR4 gene. A Haploview software package was used to analyze the haplotypes.</p><p><b>RESULTS</b>SNPs rs1927911 and rs2149356 were associated with LAA stroke. Genotypic and allelic frequencies of rs1927914 did not differ significantly between the two groups. Genetic variants of the three SNPs did not vary significantly between all subsets. Haplotype analysis was revealed a significant difference between the LAA group and the control group. Compared with the controls, the frequencies of haplotypes H2 and H8 were lower, and that of H3 was greater in the LAA group.</p><p><b>CONCLUSION</b>An association between the TLR4 gene polymorphisms and LAA stroke subtype in ethnic Han population in northern China has been found. However, no association of liability to atherosclerosis in different vascular bed has been found with these polymorphisms.</p>
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Povo Asiático , Etnologia , Genética , Sequência de Bases , Doença da Artéria Coronariana , Etnologia , Genética , Patologia , Vasos Coronários , Patologia , Estudos de Associação Genética , Dados de Sequência Molecular , Polimorfismo de Nucleotídeo Único , Acidente Vascular Cerebral , Etnologia , Genética , Patologia , Receptor 4 Toll-Like , GenéticaRESUMO
MicroRNAs (miRNAs) are endogenous,small,non-coding,single-stranded RNA molecules,which can specifically bind to the 3'-terminal non-coding regions of mRNA chain to induce posttranscriptional gene silencing effect and regulate the expression of mRNA.Recent studies have shown that miRNAs have extensively involved in various pathophysiological processes,including atherosclerosis,cerebral ischemia and hypoxia tolerance,cerebral edema,neuronal cell death,suggesting miRNAs may be used as biological markers of the early diagnosis of ischemic stroke and the biological target of early treatment.This article reviews the biological characteristics of miRNAs,its relationship with ischemic stroke and mechanism of action in order to provide new evidence and ideas for miRNAs in the diagnosis and treatment of ischemic stroke.