RESUMO
Objective To establish a HPLC method for determining the content of paeoniflorin in Dahuang Zhechong Tablets. Methods Stationary phase was C18 column (4.6 mm?250 mm, 5 ?m), mobile phase was acetonitrice-0.1% phosphoric (14∶86), detection wavelength was 230 nm, flow rate was 1.0 mL/min and column temperature was 30 ℃. Results There was good linear relationship between the concentration of paeoniflorin and area integral value in the range of 0.10～0.90 ?g, r =0.999 7. The average recovery was 99.98% with RSD=0.81% (n =6). Conclusion This method is simple, accurate, with good reproducibility and high precision, and can be used to control the quality of Dahuang Zhechong Tablets effectively.
RESUMO
Objective:To study the effects of cyclosporine A coadministrated with azathioprine,mycophenolate, mizorihine,rapamycin and/or prednisone on liver function in renal transplant recipients.Method:The drug history records of 600 renal transplant recipients in 1995 to 2005 were retrospectively investigated.Biochemical indexes before and after the treatment with cyclosporine A coadministrated with other immunosuppressants were analyzed.Result:The liver damage was found in 109 cases(18.2%)among 600 cases.The blood concentrations of cyclosporine A in the group with abnormal liver functions were significantly higher than those in the group with normal liver functions(P
RESUMO
Objective To establish the method for determination of caffeic acid in Yinpujiedu Tablets by HPLC. Methods Stationary phase was Lichrospher-C18 (4.6 mm?250 mm, 5 ?m), mobile phase was methanol-0.1% phosphoric (26:74), detection wavelength was 323 nm, flow rate was 1.0 mL/min and column temperature was 30 ℃. Results The calibration curve were linear in the range of 0.778～2.72 ?g for caffeic acid (r=0.999 9). The average recovery rate was 98.80%, RSD was 0.56%. Conclusion This method is simple and convenient for the operation. It is very accurate and suitable for the quality control of Yinpujiedu Tablets.