RESUMO
Cystatin, a cysteine protease inhibitor found in many parasites, plays important roles in immune evasion. This study analyzed the molecular characteristics of a cystatin from Fasciola hepatica (FhCystatin) and expressed recombinant FhCystatin (rFhcystatin) to investigate the immune modulatory effects on lipopolysaccharide-induced proliferation, migration, cytokine secretion, nitric oxide (NO) production, and apoptosis in mouse macrophages. The FhCystatin gene encoded 116 amino acids and contained a conserved cystatin-like domain. rFhCystatin significantly inhibited the activity of cathepsin B. rFhCystatin bound to the surface of mouse RAW264.7 cells, significantly inhibited cell proliferation and promoted apoptosis. Moreover, rFhCystatin inhibited the expression of cellular nitric oxide, interleukin-6, and tumor necrosis factor-α, and promoted the expression of transforming growth factor-β and interleukin-10. These results showed that FhCystatin played an important role in regulating the activity of mouse macrophages. Our findings provide new insights into mechanisms underlying the immune evasion and contribute to the exploration of potential targets for the development of new drug to control F. hepatica infection.
RESUMO
Objectives:To evaluate the effect of hepatic VX2 tumor mode treated by radiofrequency ablation(RFA),external high frequency thermotherapy(HFT) and transcatheter arterial chemoembolization(TACE).Methods:we replicated the model of hepatic tumor via transplantation and treated it with RFA,TACE and HFT.All the experimental rabbits was divided into four groups based on the different combination:group A:RFA+TACE+HFT,group B:RFA+HFT,group C:FA+TACE,Group D:TACE+HFT.Results:(1)After treatment,ALT and AST rised obviously on the first day in all groups,especially group A,which recovered the most slowly.In group B,however,ALT and AST was the lowest on the first day but decreased to normal most rapidly(P