RESUMO
Background: Escherichia coli is one of the most frequent causes of many bacterial infections, including Urinary Tract Infections (UTI), blood stream infections, otitis media, pneumonia, meningitis, traveler’s diarrhoea, enteric infections and systemic infections. This study was done with the aim to surveying antibiotic sensitivity pattern of isolated Escherichia coli in both sex attended in NIMS Hospital, Jaipur under the taken time period.Methods: In this cross-sectional study, 62 Escherichia coli were isolated from various clinical specimens of the patients attending both OPD and IPD. The strains were selected using the laboratory standard methods and culture-specific. The antibiotic susceptibility testing was performed using Kirby-Bauer disk diffusion method.Results: Out of total 62 isolates of Escherichia coli 26(41.93%) isolates were from male while 36(58.064%) from female patients. Maximum sensitivity were shown by Polymyxin B and Colistin i.c 100% followed by Nitrofuratonin 82.5% followed by Meropenem 79.03%, Aztreonam 72.58%, Piperacillin/ Tazobactam and Ciprofloxacin 61.30%, each Amikacin 56.45%, Imipenem 54.83%, Ofloxacin 45.16%, Cefepime 43.54%, Ceftazidime 38.71%, Gentamycin and Ceftriaxone 37.09% each, Cefotaxime 30.64%, Norfloxacin 27.5%. Maximum resistance shown against Norfloxacin 72.5%, followed by Gentamycin and Ceftriaxone 62.90%, Ceftazidime 61.30%.Conclusions: Escherichia coli infected more in urinary tract infection as compare to other sample in human, and it is common in female than male. Regular monitoring of antimicrobial susceptibility for E.coli is recommended to improve treatment. A changing trend in antibiotic sensitivity profile of the isolates need to be monitored as there is limited availability of newer drugs and the emergence of resistant bacteria far exceeds the rate of new drug development.
RESUMO
Introduction: In the recent few decades, there had beena dramatic rise in the global incidence of dengue. As thedisease is associated with high mortality and morbidity, arapid and accurate diagnosis is essential for early appropriatemanagement and for prevention of complications. Now days,a variety of rapid diagnostic tests (RDTs) kits and EnzymeLinked Immuno Sorbent Assay (ELISA) based test kits areavailable. In this present study we have attempted to doa diagnostic test evaluation of rapid ICT with ELISA fordetection of NS1 antigen and IgM antibody for acute denguediagnosis.Material and Methods: A Cross-Sectional study was carriedout in the Department of Microbiology, Govt. MedicalCollege, Raigarh from November 2017 to October 2018. 1200suspected serum samples were tested for dengue identificationby Immuno-chromatography (ICT) based RDT kit (J. Mitraand Co. Pvt.Ltd, India) which detects NS1 antigen, IgM andIgG antibodies. From the Dengue rapid reactive samples testdone by ICTs were subjected to ELISA tests for ConfirmationNS1 antigen and IgM antibodies.Results: The Rapid Dengue Test showed a sensitivity andspecificity of 98% and 74% for NS1 antigen detection and76% and 90% for IgM Antibody detection.Conclusion: Good sensitivity and specificity of rapiddiagnostic tests for early detection of dengue was observed.These kits are suitable for early detection of dengue cases,as with high sensitivity and specificity it can help in earlyscreening of patients and can further limit the spread ofdisease where ELISA facilities are not available