RESUMO
Effect of genistein, a protein tyrosine kinase inhibitor, on TAME-esterase induced contractions were studied on rat aorta strips in vitro. Data showed that TAME-esterase induced contractions were concentration dependent and these contractions were significantly inhibited when rat aorta strips were pre-incubated with genistein. The present findings provide evidence for the possible contribution of tyrosine kinases during TAME-esterase induced contractions in aorta.
Assuntos
Animais , Aorta/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Genisteína/farmacologia , Masculino , Contração Muscular/efeitos dos fármacos , Peptídeo Hidrolases/fisiologia , Proteínas Tirosina Quinases/antagonistas & inibidores , Ratos , Ratos Sprague-DawleyRESUMO
Contractions induced by TAME-esterase on rat aorta strips mounted in vitro were significantly inhibited in presence of Vitamin C. The work lends support to the role of ascorbic acid in preventing endothelial dysfunction through release of nitric oxide. It is suggested that conclusions TAME-esterase could be an important biological marker associated with onset of vascular discases such as hypertension.
Assuntos
Animais , Aorta Torácica/efeitos dos fármacos , Ácido Ascórbico/farmacologia , Endotélio Vascular/efeitos dos fármacos , Contração Muscular/efeitos dos fármacos , Peptídeo Hidrolases/farmacologia , Ratos , Ratos Sprague-DawleyRESUMO
Possible pharmacological effects of N-alpha-tosyl L-arginine methyl ester [TAME] were studied on rat aorta strips in vitro. Results showed that [TAME]-esterase was an endothelium dependent component that involved a nitric oxide cyclic-GMP mediated pathway. Furthermore, during activation of Kinin-Kallikrein system, TAME-esterase induced contractions involve degradation of kinins by kininases.