RESUMO
Objectives : To determine the levels of circulating endothelial cell soluble adhesion molecules, sP-selectin, sL-selectin and sE-selectin, vascular cell adhesion molecule-1 [sVCAM-1], intercellular adhesion molecule-1 [sICAM-1] and platelet endothelial cell adhesion molecule-1 [sPECAM-1] in diabetic pregnant women with and without preeclampsia
Patients and Methods: The study population was composed of 10 healthy non-pregnant women, 20 healthy pregnant women, 30 diabetic pregnant women and 30 diabetic pregnant women with preeclampsia Concentrations of soluble adhesion molecules were determined by ELISA
Results: Normal pregnancy was associated with a significant increase in maternal plasma concentration of sP-selectin, a decrease in sL-selectin, and no change in the levels of sE-selectin, sVCAM-1, sICAM-1 and sPECAM-1 when compared with the corresponding levels of non-pregnant controls. Diabetic pregnant women without preeclampsia revealed significant increase in sP-selectin, sE-selectin and sVCAM-1, a decrease in sL-selectin, but no change in sICAM-1 and sPECAM-1 levels compared to pregnant controls. While, all endothelial cell adhesion molecules were significantly elevated in diabetic pregnant women with preeclampsia, except sL-selectin which showed a significant decrease
Conclusions: Increased concentration of sP-selectin and decreased sL-selectin, as well as the lack of change in endothelial cell-associated soluble adhesion molecules suggests that normal pregnancy is associated with platelet and leukocyte activation, but not endothelial cell activation. Elevated circulating levels of sVCAM-1, sICAM-1 and sPECAM-1 and sE-selectins indicate endothelial activation and/or damage in preeclampsia
RESUMO
Objective: To evaluate the role of paraoxonase mylesterase [PON-1], interleukin-6 [IL-6] and. plasminogen activator inhibitor-1 [PAI-1] in the pathogenesis of cardiovascular complications in diabetic patients
Patients and Methods: This study included 17 healthy subjects, as control group, 24 diabetic patients [DM group] and 27 diabetic patients with acute coronary diseases [DM+CVS group]. The levels of blood glucose, whole blood glycated hemoglobin [HbA1c], triglycerides [TG], total cholesterol [TC], high density lipoprotein cholesterol [HDL-C] and PON -1 activity were measured Spectrophotometrically. Low density lipoprotein cholesterol [LDL-C] was calculated. PAI-1 and IL6 levels were assayed by ELISA
Results: PON-1 arylesterase activity was significantly decreased in DM and DM+CVS groups [108.81 +/- 9.44 U/ml and 86.86 +/- 12.44 U/ml, respectively], compared to control group [123. 49+/- 13.72 U/ml] at p<0.05. On the other hand, the IL-6 and PAI-1 levels were signiticantly increased in DM [27. 65+/-6. 1 pg/ml;14. 66+/-1. 88 ng/ml] and in DM+CVS groups [38. 32+/- 8. 9 pg/ml 16. 31 +/- 2. 69 ng/ml] compared to control group [12.32 +/- 1.38 pg/ml, 12.46 +/- 1.124 ng/ml] respectively at p<0.05. The PON-1 activity was significantly inversely correlated with glucose, TC and LDL-C levels and positively correlated with HDL-CE. IL-6 and PAI-1 were positively correlated with glucose, TG and TC. Furthermore, IL-6 was found to be correlated negatively with PON-1 [r=0.455, p<0.05] and positively with PAI-1 [r=0.479, p<0.05]
Conclusions: PON-1, PAH and IL-6 levels have significant roles in the pathogenesis of atherosclerosis complications of diabetes mellitus and they are involved with lipoproteins in the Complex process of atherosclerosis that leads to acute coronary syndromes