RESUMO
It behooves the researchers to make attempts to conduct more studies; for, it deems crucial to utilize more up - to - date technologies in some special fields as cloning and producing trans genetic animals. These methods are supposed to proliferate the production of animals which are persistent against diseases, more milk production and pharmaceutical proteins. The aim of this study was in vitro production and verification of bovine embryo for next researches. After maturation and fertilization of oocyts, embryos Cultured in SOF medium at 39c temperature and%5 co2 in incubator for 8 day. Then 200 high quality blastocyst selected and vitrified [%40 ethylene glycol,%18 focal, 13 molar sacarose]. After thawing, embryos cullored for 24 hours and viability of them assessed by re-expanded embryos.%75 of oocyts fertilized and cleavaged,%20 of fertilized oocyts recived to blastocyst stage, percentage of post - thaw re - expanded blastocysts for 7 day and 8 day was%65 and%70 respectively. Even though post - thaw survival blastocyst rate on 7 day was higher than the 8 day, but difference between the two groups not significant. Rate of Fertilized oocyts, developed blastocysts and blastocysts survival after verification was as same as the other countries researchers