RESUMO
Background: One of the new methods to overcome problems with using essential oils [such as instability, evaporation and decomposition in the environmental conditions[ in pharmaceutical, food and agricultural industries is use of Solid Lipid Nanoparticles [SLNs] carrier systems
Objective: Preparation of SLNs containing the Zataria multifloni Boiss essential oil. Methods: In this regard, an experiment was performed on the preparation of SLNs containing the Zataria multiflora Boiss essential oil at Nanotechnology Research Center of School of Pharmacy, Mashhad University of Medical Sciences. SLNs containing Zataria multiflora Boiss essential oil was prepared using high tensile pressure homogenization and ultrasound
Components of SLNs include lipids, Zataria multiflora Boiss essential in fat phase and poloxamers 188 in the aqueous phase
The mean particle size and zeta potential, essential oil encapsulation percentage and thermal analysis were measured using particle size analysis instruments, gas chromatography and differential scanning calorimetry [DSC], respectively. Moreover, an electron microscope was used for imaging of SLNs
Results: The results showed that particle size, polydispersity index and zeta potential of the above formulations were respectively about 486 nm, 0.296, -27.2 mv. The results obtained from transmission electron microscopy [TEM] showed particle size less than 300 nm and particles were spherical
Thermal analysis by DSC, confirmed the presence of solid particles in the prepared SLN. Also, the essential oil encapsulation percentage was 95.2 percent. Stability studies of particle size and zeta in four months showed SLNs containing essential oils had relatively good stability
Conclusion: In general, the results of the present research showed that SLNs composed of stearic acid, was good carriers for Zataria multifloni Boiss essential oil
RESUMO
Teucrium podium L. is belong to the Labiatae family which is used for medical porpose. There are several reports about the antidiabetic, antispasmodic, antibacterial and blood lipid and pressure lowering properties of Teucrium podium L. The aim of this study is investigating the effects of aqueous-ethanol extract of arial part of Teucrium polium on rabbit's mean arteial blood pressure, heart rate and intraventricular pressure in vivo. Twenty four New Zealand White rabbits weighed [2-3 kg] were randomly divided into four groups. Animals were anesthetized by sodium thiopental [50 mg/kg, ip] and after teracheostomy, jugular vein and carotid artery were cannulated. The extract doses[20, 40 and 80 mg/kg] were injected via jugular vein and blood pressure, heart rate and intraventricular pressure were measured by pressure transducer which was connected to power lab. The data was expressed as Mean +/- SEM, and p<0.05 was considered significant. The extract reduced blood pressure but it was not significant. The extract had no effect on heart rate. The extract increased Max dp/dt, maximum left ventricular pressure [LVP[max]] and decreased Min dp/dt significantly but had no effect on minimum left ventricular pressure [LVP[min]]. The results show the extract has a positive inotropic effect on the heart. The extract reduces blood pressure but this effect is counter balance by the inotropic effect of the extract. The extract has no effect on the heart rate