RESUMO
Objective: to evaluate matrix metalloproteinase-9 [MMP-9], inhibitor of metalloproteinase-1 [TIMP-I], sFas, hepatocyte growth factor [HGF] and myeloperoxidase [MPO] levels in newly diagnosed patients with acute leukemias and to correlate their levels with the patients' clinical data. We also tried to study the correlation between apoptosis and angiogenesis in acute leukemias
Patients and Methods: this study included 15 patients with acute lymphoblastic leukemia [ALL]. 15 patients with acute myeloblastic leukemia [AML] and 15 healthy persons of matched age and sex served as controls. The levels of serum human total matrix metailoproteinase-9 [MMP-9], human tissue inhibitor of metalloproteinase-1 [TIMP-I], human sFas and plasma concentration of hepatocyte growth factor [HGF] were determined by enzyme linked immunosorbent assay. The measurement of MPO was done in peripheral blood polymorphonuclear layer
Results: elevated levels of the angiogenic factors [MMP-9. its inhibitor TIMP-I, and HGF] as well as the apoptotic factor [sFas] were detected in both acute leukemic groups when compared with controls while MPO was significantly decreased in both groups as compared to controls. Significantly higher levels of MMP-9 and MPO were present in AML group than ALL group while sFas level was significantly higher in ALL than AML group. HGF level was insignificantly different between both groups. Higher values of MMP-9, HGF and sFas were observed in M4 and M5 than other FAB subtypes and in adult than child ALL. No correlation was present between angiogenesis and apoptosis in acute leukemic patients
Conclusion: angiogenesis and apoptosis are independent processes in acute leukemias