[Evaluation of induced apoptosis of apigenin in 3 lines of human B cell lymphoma in vitro]

Apoptosis or programmed cell death [PCD] is an important mechanism in both development and homeostasis of human tissues for the removal of superfluous cells and its induction is an effective method in the treatment of cancer. The aim of this study was the evaluation of induced apoptosis of apigenin in the human B cell lymphoma. In this experimental study, three human lymphoma B cells were cultured in RPMI1610, supplemented with 10% fetal calf serum, peniciline-streptomycin and L-glutamine, at 37°C for 2 days. Cancer cell lines were treated by apigenin and Cellular vital capacity was determined by MTT. Then, effects of apigenen on human lymphoma B cells were examined by flowcytometry technique. During MTT, human lymphoma B cell line revealed significant apoptosis at 10, 15 and 20 micro g/ml concentrations compared with controls [p<0.01]. Flowcytometry assay showed that apoptotic bodies were significantly different in three human lymphoma B cells and 48 hours was appropriate time for inducing apoptosis. This study revealed the anticancer effects of apigenin and its effect on apoptosis on human lymphoma B cells in vitro

Comparing the effects of crystalloid with ephedrine+ crystalloid on maternal hypertension in elective cesarean section under spinal anesthesia

Background and Purpose: Pretreatment with crystalloid has not been able to maintain blood pressure during elective cesarean section under spinal anesthesia, and it seems that prophylactic ephedrine+crystalloid can reduce hypotension and administration of high doses of ephedrine. The aim of the present study is to compare the effects of ephedrine+crystalloid pretreatment with crystalloid alone on blood pressure during elective caesarean section under spinal anesthesia

Methods and Materials: In this double blind clinical trial, we enrolled 72 women admitted to Ali-ebne-Abitaleb hospital in Zahedan, Iran, who were candidates for elective caesarean section under spinal anesthesia in 2006,and divided them into group A [ephedrine+cistalloid] and group B [placebo+crystalloid] by convenience sampling. Infusion with 10ml/Kg of ringer solution was made within 10-15min before the initiation of spinal block in both groups. Also, 10mg ephedrine in group A and placebo in group B were added to the solution. After newborn delivery, 20 IU oxitocin and 2mg midazolam were administered to all patients. Systolic BP, HR, nausea, vomiting, level of spinal block and neonatal Apgar were recorded and analyzed by Chi-square and student's t-tests [P<0.05 was considered as statistically significant]

Results: Episode of hypotension occurred in 25% of group A and 55.6% of group B patients, suggesting a statistically significant difference between the two groups [P=0.008]. However, there were no significant differences between the two groups in their mean systolic BP after intervention [105.61 +/- 7.13 in group B versus 107.89+/-9.84 in group A, in 1-10min; also, 106.20+/-8.52 in group B versus 109.39 +/- 6.72 in group A, in 11-60min]

Conclusion: Prophylactic infusion of ephedrine+crystalloid is more effective than crystalloid pre-hydration in the prevention of hypotension during spinal anesthesia for elective caesarean section