RESUMO
A simple liquied chromatographic method for the simultaneous determination of ampicillin and cloxacillin in drug formulations is presented. The method could be applied for the determination of the two drug substsnces in presence of their degradation products without apparent interferences. The LC-sparation was undertaken on a reversed phase 100 RP-18 Lic hrospher [5 micro m] column using a mobile phase of acetonitrile and 1% acetic acid, 51:49, v/v at a rate of 1.8 ml/min with UV detection at 240 nm. The suitability of the LC-system developed, as tested by replicate injections of the sample and standard preparations, gave relative standard deviations [RSD[S]] less than 2%. The statistical evaluations of the results of the assay and recovery experiments indicated good accuracy of the proposed LC-method