Effect of different temperatures on viability of seven encysted metacercariae recovered from freshwater fishes in Qualyobia, Egypt

Encysted metacercariae [EMC] from seven trematod-zoonotic parasites were exposed to different temperature mechanisms. Boiling of the infected fishes was sufficient to kill the EMC, frying of fishes for five minutes was quite sufficient to inhibit the viability of EMC, but frying for 10 minutes killed all EMC. Grilling of infected Tilapia zillii was sufficient to kill EMC after 10 minutes; however five minutes were sufficient only to kill EMC in Clarias gariepinus. Regarding chilling at 5°C, T. zillii EMC showed variation in response. Complete loss of viability of Prohemistomatidae EMC was achieved after 14 days, for Haplorchidae after 11 days, for Diplostomatidae after 12 days, while Clinostomatidae EMC required 15 days. For Cl. gariepinus, Bagrus bajad and Chrysichthys auratus achieved results were similar to those for T. zillii but with fewer days of withstanding chilling. The EMC infecting Tilapia lost their viability by freezing at -5 °C and -10 °C for Prohemistomatidae after 48 and 40 hours, for Diplostomatidae after 24 and 16 hours and for Clinostomatidae cysts after 48 and 32 hours respectively. In infected Clarias gariepinus, Bagrus bajad and Chrysichthys auratus the EMC lost their viability by fireezing at -5 °C and -10 °C for periods shorter than those of Tilapia sp

Metacercariae recovered from fresh-water fishes in the vicinity of Qualkyobia Governorate, Egypt

A total of 410 Tilapia zillii, 70 Clarias gariepinus, 30 Bagrus bajad and 15 Chlrysichthys auratus fishes were collected from different water bodies in Qualyobia Governorate. Microscopic examination of samples showed infection in muscles and head region [gills and branchial cavity] with encysted metacercariae in 91.7% of T. zillii, 82.85% of Cl. gariepinus, 70% of B. bajad and 86.66% of Ch. auratus. The highest distribution of metacercarial infection among the T. zillii was in the muscles of posterior third and tail followed by the middle third, anterior third and head region [gills and branchial cavity] while the highest distribution of metacercarial infection among Cl. gariepinus was in the middle third muscles followed by the posterior third, anterior third and lastly in the head region. The highest metacercarial infection among B. bajad and Ch. auratus fish was in the posterior third muscles and branchial cavity respectively. The taxonomic morphology of the encysted metacercariae and the excysted ones was given

Leukaemogenic effect of toxoplasmosis and irradiation on albino rats

leukaemogenic effect of irradiation associated with toxoplasmosis was studied. Rats were infected with Toxoplasma gondii RH strain 3 weeks prior to irradiation. The leukaemic effect of irradiation was aggravated by toxoplasmosis as leukaemia appeared in 10% of 300 cGy irradiated rats and in 30% of 500 cGy irradiated ones, six months after irradiation

study of the rectal snip transparency and kato techniques in diagnosis of intestinal schistosomiasis

A comparative study of the rectal snip transparency and Kato techniques revealed that both methods are highly sensitive in the diagnosis of intestinal schistosomiasis. Nevertheless, it was concluded that any of them is not sufficient alone especially in cases intended to assess cure or activity of the disease