Polyacrylamide gel electrophoretic pattern of urinary and serum proteins in some chronic renal diseases

In this study, urinary and serum proteins in control, CRF and nephrotic groups are fractionated into 11 fractions arranged from the anodal side on polyacrylamide gel electrophoressis. Statistical analysis of the results revealed that: 1- In chronic renal failure group, both quantitative and qualitative changes in serum and urinary proteins occur. There is a very highly significant increase in urinary total proteins, F1[1] F[5] and F[6] as well as a highly significant increase in urinary F4 and a significant increase in urinary F[2]. On the other hand there is a highly significant decrease in serum total proteins and F6. Also, there is a highly significant decrease in F[2] and F[6]. On the other hand, there is a highIy significant increase in serum F1 and F9. F10 and F11 are present only in CRF group but not in control or nephrotic groups. 2- In nephrotic group, there is a very highly significant increase in urinary total proteins, F1, F3, F5 and F6 as well as a highly significant increase in F[2]. The urinary protein F7, F8 and F9 are present in nephrotic group but F[10] and F[11] are absent. As for serum, there is a very highly significant decrease in serum total proteins. F[2] and F[6]. a highly significant decrease in F[5] and a significant increase in F9 and absence of F[10] and F11 which are present in CRF group only. 3 In CRF group, there is a highly significant increase in serum F 1, which may be B2 microglobulin and may be of value in the genesis of symptoms in CRF. 4 Considering urinary F3, which is most probably transferrin, it showed a very highly significant increase in nephrotic group, but insignificant change in CRF group. So, it may be considered as an indicator for pathological glomerular proteinuria. 5 As regards urinary F4 which is most probably B-globulin, it showed a highly significant increase in CRF group but insignificant change in nephrotic group. This may be of value in diagnosis and differntiation between CRF and nephrotic syndrome.6- F7, F8 and F9 are most probably IgM. These fractions are present in urine of CRF and nephrotic group, but absent in urine of control group because they can not pass through the normal glomerular membrane. 7- There is a highly significant increase in serum F9 in CRF group. This fraction is of a large molecular weight and essentially unfilterable and may have a role in the genesis of renal failure symptoms. It is concluded that fraction of urinary as well as serum proteins by polyacrylamide gel electrophoresis may be of high value in diagnosis and prognosis of renal disease status. The increase in urinary F3 is an indicator of glomerular proteinuria which occurs in nephrotic syndrome and the increase in urinary F4 is an indicator of tubular proteinuria and may be of diagnostic value in chronic renal failure

Effect of exogenous calcium ions on prostaglandin output from rat hepatic tissue slices

This vitro study was an attempt to study the possible role of Ca[++] ions on metabolism of prostaglandins in hepatic tissue of albino rats. Addition of calcium ions in the form of calcium gluconate 10% ampule in a dose of 29 ug/gm wet tissues to the Kreb's Ringer phosphate media cause a high significant increasre in PGE[2] output from hepatic tissue slices. This is associated with a significant decrease in PGF[2]. These fingings may give an additional information of the possible role of Ca[++] ions on catabolism of prostaglandins. From the present study, it is concluded that addition of extracellular Ca[++] ions to the incubation media resulted in increase PGE2 synthesis and efflux with decrease PGE2 synthesis and efflux from hepatioc tissue of albino rats. The effect of Ca[++] ions on prostaglandins synthesis and release must be put in mind on using drugs which alter PG synthesis, efflux or both. An example of these drugs are calcium channel blockers, beta adrenergic receptor stimulator as well as alpha adrenergic receptor stimulator, which are commonly used in the treatment of many cardiovascular diseases. Their use must be put under observation to avoid undesirable effects as a result of increase or decrease prostaglandins formation

Biochemical effects of some commonly used legumes on alloxan diabetic rats

The present work was undertaken in an attempt to investigate the effect of long use of some selected legumes on fasting blood glucose level and blood lipid pattern in alloxan diabetic rats. Feeding diet containing termis and helba to diabetic rats for one month induced significant decrease in fasting blood glucose, total cholesterol, triglycerides and low density lipoprotein levels with a high significant increase in high density lipoprotein level. From the present study, it is concluded that eating termis and helba mixture before breakfast every morning leads to improvement of fasting blood glucose level and blood lipid pattern of alloxan diabetic rats. And it is a good habit for the diabetic patients to take a mixture of termis and helba everymorning before breakfast