Using some recent techniques in diagnosis of some zoonotic bacterial diseases transmitted through milk

A total of 200 different samples [100 raw milk, 50 Kareish cheese and 50 yogurts] were collected from dairy shops, street venders and supermarkets from different localities in Alexandria Province to be investigated for presence of bacterial pathogens and to evaluate plasmid profiling as a tool for detection of bacteria transmitted through milk. The results of bacteriological examination firstly revealed that the rates of isolation of E. coli were 18, 26 and 18% from raw milk, kariesh cheese and yoghurt samples, respectively; secondly, results revealed that the rates of isolation of Salmonella species were 5, 6 and 2% from raw mill, kariesh cheese and yoghurt samples, respectively and finally they clarified that the rates of isolation of Staph. aureus were 44, 34 and 18% from raw milk, kariesh cheese and yoghurt samples, respectively. Serological identification of E. coli and Salmonella spp. was carried out. The public health significance of isolated bacteria and the diagnostic value of plasmid were discussed

Serological investigation of salmonellosis among some wild birds and human contacts

A total of 311 serum samples were collected from wild birds and their human contacts [211 from wild birds including gray heron, wild ducks, wild fowl, wild quail and wild captive birds and 100 from their human contacts]. All collected samples were subjected of serological response to Salmonella Typhimurium [ST] and Salmonella Entritidis [SE] by using the Widal and Felix test kits [ST antigen TMH code 63542] and [SE antigen ENH code 63272] supplied by Bio-Rad Laboratories. It was found that the overall percentage of Salmonella antibodies of wild birds was 13.7% [8.5 and 5.2% for ST and SE respectively]. On the other side, the overall seropositive percentage of human salmonellosis in examined serum samples of the human contacts was 10% and SE was most prevalent serotype affecting human contacts with wild birds. The effect of some epidemiological factors including sex and seasons of the year on the occurrence of salmonellosis in both wild birds and their human contacts were studied. The obtained results clarified that salmonellosis incidence was increased in males than in females with highest seasonal incidence occurring in winter season in both wild birds and human. The public health importance of Salmonellosis was discussed

Prevalence of bovine infection with mycobacterium bovis some Egyptian Governorates

One hundred and thirty seven [4.6%] out of 3000 cattle at different ages from seven governorates of Egypt [Qualubia, Sharkia; Gharbia, Giza, Behira, Sohag and Ismailia] were found positive for tuberculosis by single inradermal [SID] tuberculin Bacteriological examination of tested samples from slaughtered reactor cattle revealed the isolation and identification of Mycobacterium bovis [M. bovis] and Mycobacterium other than tuberculosis [MOTT] in 90 [65.7%] and 7 [5.1%] samples, respectively. One hundred and eight [78.83%] serum samples out of 137 tuberculin reactors were found positive for enzyme linked immunosorbent assay [ELISA] by using test. Post mortem [PM] examination of slaughtered reactor cattle revealed tuberculous visible lesions [VL] and non-VL [NVL] in 94 [68.6%] and 43 [31.4%] animals, respectively. Tuberculous lesions were observed in 41 29.9%], 19[13.9%], 20 [15%] and 14 [10.2%] in 137 slaughtered reactor cattle with pulmonary, digestive, mixed and generalized VL, respectively. M.bovis synthetic early secretory antigenic target 6 peptide antigen [ESAT6-p]. Polymerase chain reaction [PCR] amplification assay was performed on selected tissues samples from 10 slaughtered tuberculin reactors with pulmonary, digestive, mixed or generalized VL, or NVL. Eight [80%] out of the 10 samples gave positive results with PCR assay. On the other side, only 6 [60%] and 7 [70%] of these samples were found positive for M.bovis isolation and ELISA, respectively. The results obtained in the present study point to the importance of the periodical tuberculin testing of animals, use of ELISA assays with M.bovis-specific antigens as a complimentary diagnostic toot to tuberculin test and application of PCR test for direct and rapid diagnosis of tuberculosis. Improvement of diagnostic assays for M.bovis may be of value in the effectiveness of test and slaughter eradication programs of bovine tuberculosis in governorates of Egypt