[Toxicity Evaluation of coral madrepora and human BMG in peripheral mononuclear blood cells]

Disadvantage of auto grafts make widespread use of other bone graft materials such as xenografts and allografts. cytotoxic effect of materials and biomaterials on Lively human cells is the Object that always should be observed. In present study had tried to comparison evaluation cytotoxic effete of two bio material -human BMG and coral madrepora -on human peripheral mono nuclear blood cells. In present experimental study after to make preparation peripheral blood with fycole method and After preparation and sterilization of human BMG and coral madrepora, Then, human BMG and coral madrepora, aside to gether are divided into 10, 20, 50 mg portions and cultured on 24-well of 100000 mononuclear cells of peripheral blood and they are evaluated after 24, 48 and 72 hours. MTT method was used for evaluation of cytotoxic effect using ELISA reader in 540 nm wave lengths. Light absorption rate depends on MTT uptake by the cells, indicating vital activity of the cells. Result evaluated with variance and whilcoxon-rank sum and mann u whitnney test. All of the human BMG doses that were used weren,t toxic and all of them increased number of the human peripheral blood mononuclear cells [P< 0.005]. But coral madrepora, was decried number of the human peripheral blood mononuclear cells after 24 hour in 50 mg doses and after 48 hours in 10 mg doses and after 72 hours in 20 and 50 mg doses. With increasing of human BMG concentration and passing time, increasing of human peripheral blood mononuclear cells can be observed and it has a statistically significant difference with control group. Therefore, as a conclusion, human BMG is compatible with human blood mononuclear cells. But coral madrepora had toxicity for this blood cells. However for more judgment, need more study with different cells

[Histomorphometric comparison of vertical bone augmentation between autogenous demineralized dentin matrix and biooss on parietal bone of rabbit]

Lack of sufficient bone is a main problem in implant treatment. Bone graft materials are potential solutions to this problem. Bioose is a grafting material that is routinely used for this purpose. In some studies autogenous demineralized dentin matrix [ADDM] has been used as bone substitute. The aim of this investigation was to compare the two grafting materials [ADDM and Biooss] on vertical bone augmentation in parietal bone of rabbits. Six white Newsland rabbit were used in this study. Two screws were inserted on the parietal bone of each rabbit in anteroposterior direction. Biooss powder was put around the anterior screw and ADDM particles around the posterior screw and both were covered with gutta percha membrane. The rabbits were sacrificed after 12 weeks and the specimens were sent for histological examination. Mann Whitney and paired T-tests were used to analyze the data. Range of bone height in Biooss group were between 0.1-2.1 and in ADDM group were 0.4-3.2 mm. Mean Bone height in Biooss group was 1.43 +/- 0.84 and in ADDM group was 1.4 +/- 1.03. Mann Whitney test showed no significant difference between the two groups in bone height. In this study the vertical bone augmentation in Biooss and ADDM groups were the same. Therefore, ADDM was an effective biomaterial for vertical bone augmentation in this study

[Evaluation of coral [madrepora] cytotoxicity in fibroblast cell culture]

Bone defect is one of the major problems facing dentists and maxillofacial surgeons. Several approaches, such as incorporating autografts, xenografts, as well as using polymers have been suggested. The calcified skeleton of corals has been proposed for bone grafting over the past few years. Toxicity is the most important concern of grafting foreign material. The purpose of this study was to investigate the toxicity of coral [madrepora] skeleton in fibroblast culture. Powdered corals with particle sizes of less than 100 micron were autoclaved. Coral powder was added to culture medium consisting of 90% RPMI, 10% FBS and different concentrations of 0.5, 1, 2, 5, 10, 20 and 50 milligrams per 100 milliliters of media were prepared. Fibroblast cells were obtained from Pasteur institute for inserting into culture mediums. Three samples were prepared from each concentration. Complete medium culture was the control. Ninety six well plate containing culture medium, fibroblasts and coral powder were prepared. They were placed into a programmed incubator containing 5% CO2 for 24, 48 and 72 hours at 37°C. The [MTT] was then added to plate culture mediums. Since light absorption has close relationship with cells viability, ELISA reader apparatus was used to measure light absorption [optic dosimeter]. The collected data was statistically analyzed using ANOVA test. Light absorption in control group was similar to light absorption in the various concentrations of coral medium culture along with incorporated fibroblasts. Based on the findings of this study, it can be concluded that coral [madrepora] has potentially no cytotoxic effect on fibroblast cells